Oxygen concentration imaging in a single living cell using phosphorescence lifetime of <font>Pt</font>-porphyrin

DOI Web Site 被引用文献4件 参考文献14件
  • Takashi Saito
    Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama, 226-8501, Japan
  • Noriyuki Asakura
    Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama, 226-8501, Japan
  • Toshiaki Kamachi
    Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama, 226-8501, Japan
  • Ichiro Okura
    Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama, 226-8501, Japan

書誌事項

公開日
2007-03
DOI
  • 10.1142/s1088424607000205
公開者
World Scientific Pub Co Pte Lt

この論文をさがす

説明

<jats:p> An oxygen concentration imaging system inside a single living cell, based on the phosphorescence lifetime, under a microscope was developed. A fluorescence microscope equipped with a pulsed Nd : YAG laser (532 nm) and a CCD camera equipped with a gated imaging intensifier was used. When the cell was incubated with the phosphorescent compound, platinum tetra-(carboxyphenyl)-porphyrin ( PtTCPP ) was incorporated and localized in the cell. As the phosphorescence intensity depends not only on the concentration of a quencher such as oxygen but also on the concentration of phosphorescent molecules, the oxygen concentration was measured by the phosphorescence lifetime. To measure the oxygen concentration image, the time dependence of the phosphorescence intensity was observed under a microscope. The phosphorescence intensity decay obeyed first order kinetics and the oxygen concentration imaging in the single cell was obtained by the lifetime of a single exponential decay curve. </jats:p>

収録刊行物

被引用文献 (4)*注記

もっと見る

参考文献 (14)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ