Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification

DOI Web Site 被引用文献3件
  • F J van Kuppeveld
    Department of Medical Microbiology, University of Nijmegen, The Netherlands.
  • J T van der Logt
    Department of Medical Microbiology, University of Nijmegen, The Netherlands.
  • A F Angulo
    Department of Medical Microbiology, University of Nijmegen, The Netherlands.
  • M J van Zoest
    Department of Medical Microbiology, University of Nijmegen, The Netherlands.
  • W G Quint
    Department of Medical Microbiology, University of Nijmegen, The Netherlands.
  • H G Niesters
    Department of Medical Microbiology, University of Nijmegen, The Netherlands.
  • J M Galama
    Department of Medical Microbiology, University of Nijmegen, The Netherlands.
  • W J Melchers
    Department of Medical Microbiology, University of Nijmegen, The Netherlands.

書誌事項

公開日
1992-08
権利情報
  • https://journals.asm.org/non-commercial-tdm-license
DOI
  • 10.1128/aem.58.8.2606-2615.1992
公開者
American Society for Microbiology

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説明

<jats:p>Systematic computer alignment of mycoplasmal 16S rRNA sequences allowed the identification of variable regions with both genus- and species-specific sequences. Species-specific sequences of Mycoplasma collis were elucidated by asymmetric amplification and dideoxynucleotide sequencing of variable regions, using primers complementary to conserved regions of 16S rRNA. Primers selected for Mycoplasma pneumoniae, M. hominis, M. fermentans, Ureaplasma urealyticum, M. pulmonis, M. arthritidis, M. neurolyticum, M. muris, and M. collis proved to be species specific in the polymerase chain reaction. The genus-specific primers reacted with all mycoplasmal species investigated as well as with members of the genera Ureaplasma, Spiroplasma, and Acholeplasma. No cross-reaction was observed with members of the closely related genera Streptococcus, Lactobacillus, Bacillus, and Clostridium or with any other microorganism tested. On the basis of the high copy number of rRNA, a highly sensitive polymerase chain reaction assay was developed in which the nucleic acid content equivalent to a single organism could be detected.</jats:p>

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