High-quality chromosome-scale assembly of the walnut ( <i>Juglans regia</i> L.) reference genome
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- Annarita Marrano
- Department of Plant Sciences, University of California , Davis, One Shields Avenue, Davis, CA 95616,
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- Monica Britton
- Bioinformatics Core Facility, Genome Center, University of California , One Shields Avenue, Davis, CA 95616,
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- Paulo A Zaini
- Department of Plant Sciences, University of California , Davis, One Shields Avenue, Davis, CA 95616,
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- Aleksey V Zimin
- Department of Biomedical Engineering, Johns Hopkins University , 720 Rutland Avenue, Baltimore, MD 21205,
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- Rachael E Workman
- Department of Biomedical Engineering, Johns Hopkins University , 720 Rutland Avenue, Baltimore, MD 21205,
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- Daniela Puiu
- Center for Computational Biology, Whiting School of Engineering, Johns Hopkins University , 3100 Wyman Park Dr., Baltimore, MD 21211,
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- Luca Bianco
- Research and Innovation Center , Fondazione Edmund Mach, Via E. Mach, 1 38010 S. Michele all'Adige (TN) 38010,
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- Erica Adele Di Pierro
- Research and Innovation Center , Fondazione Edmund Mach, Via E. Mach, 1 38010 S. Michele all'Adige (TN) 38010,
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- Brian J Allen
- Department of Plant Sciences, University of California , Davis, One Shields Avenue, Davis, CA 95616,
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- Sandeep Chakraborty
- Department of Plant Sciences, University of California , Davis, One Shields Avenue, Davis, CA 95616,
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- Michela Troggio
- Research and Innovation Center , Fondazione Edmund Mach, Via E. Mach, 1 38010 S. Michele all'Adige (TN) 38010,
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- Charles A Leslie
- Department of Plant Sciences, University of California , Davis, One Shields Avenue, Davis, CA 95616,
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- Winston Timp
- Department of Biomedical Engineering, Johns Hopkins University , 720 Rutland Avenue, Baltimore, MD 21205,
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- Abhaya Dandekar
- Department of Plant Sciences, University of California , Davis, One Shields Avenue, Davis, CA 95616,
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- Steven L Salzberg
- Department of Biomedical Engineering, Johns Hopkins University , 720 Rutland Avenue, Baltimore, MD 21205,
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- David B Neale
- Department of Plant Sciences, University of California , Davis, One Shields Avenue, Davis, CA 95616,
書誌事項
- 公開日
- 2020-05-01
- 権利情報
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- http://creativecommons.org/licenses/by/4.0/
- DOI
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- 10.1093/gigascience/giaa050
- 公開者
- Oxford University Press (OUP)
説明
<jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>The release of the first reference genome of walnut (Juglans regia L.) enabled many achievements in the characterization of walnut genetic and functional variation. However, it is highly fragmented, preventing the integration of genetic, transcriptomic, and proteomic information to fully elucidate walnut biological processes.</jats:p> </jats:sec> <jats:sec> <jats:title>Findings</jats:title> <jats:p>Here, we report the new chromosome-scale assembly of the walnut reference genome (Chandler v2.0) obtained by combining Oxford Nanopore long-read sequencing with chromosome conformation capture (Hi-C) technology. Relative to the previous reference genome, the new assembly features an 84.4-fold increase in N50 size, with the 16 chromosomal pseudomolecules assembled and representing 95% of its total length. Using full-length transcripts from single-molecule real-time sequencing, we predicted 37,554 gene models, with a mean gene length higher than the previous gene annotations. Most of the new protein-coding genes (90%) present both start and stop codons, which represents a significant improvement compared with Chandler v1.0 (only 48%). We then tested the potential impact of the new chromosome-level genome on different areas of walnut research. By studying the proteome changes occurring during male flower development, we observed that the virtual proteome obtained from Chandler v2.0 presents fewer artifacts than the previous reference genome, enabling the identification of a new potential pollen allergen in walnut. Also, the new chromosome-scale genome facilitates in-depth studies of intraspecies genetic diversity by revealing previously undetected autozygous regions in Chandler, likely resulting from inbreeding, and 195 genomic regions highly differentiated between Western and Eastern walnut cultivars.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusion</jats:title> <jats:p>Overall, Chandler v2.0 will serve as a valuable resource to better understand and explore walnut biology.</jats:p> </jats:sec>
収録刊行物
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- GigaScience
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GigaScience 9 (5), 2020-05-01
Oxford University Press (OUP)
