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- Gregory Arcuino
- Department of Cell Biology, Anatomy, and Pathology, New York Medical College, Valhalla, NY 10595
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- Jane H.-C. Lin
- Department of Cell Biology, Anatomy, and Pathology, New York Medical College, Valhalla, NY 10595
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- Takahiro Takano
- Department of Cell Biology, Anatomy, and Pathology, New York Medical College, Valhalla, NY 10595
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- Collins Liu
- Department of Cell Biology, Anatomy, and Pathology, New York Medical College, Valhalla, NY 10595
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- Li Jiang
- Department of Cell Biology, Anatomy, and Pathology, New York Medical College, Valhalla, NY 10595
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- Qun Gao
- Department of Cell Biology, Anatomy, and Pathology, New York Medical College, Valhalla, NY 10595
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- Jian Kang
- Department of Cell Biology, Anatomy, and Pathology, New York Medical College, Valhalla, NY 10595
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- Maiken Nedergaard
- Department of Cell Biology, Anatomy, and Pathology, New York Medical College, Valhalla, NY 10595
書誌事項
- 公開日
- 2002-07-03
- DOI
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- 10.1073/pnas.152588599
- 公開者
- Proceedings of the National Academy of Sciences
この論文をさがす
説明
<jats:p>Calcium signaling, manifested as intercellular waves of rising cytosolic calcium, is, in many cell types, the result of calcium-induced secretion of ATP and activation of purinergic receptors. The mechanism by which ATP is released has hitherto not been established. Here, we show by real-time bioluminescence imaging that ATP efflux is not uniform across a field of cells but is restricted to brief, abrupt point-source bursts. The ATP bursts emanate from single cells and manifest the transient opening of nonselective membrane channels, which admits fluorescent indicators of ≤1.5 kDa. These observations challenge the existence of regenerative ATP release, because ATP efflux is finite and restricted to a point source. Transient efflux of cytosolic nucleotides from a subset of cells may represent a conserved pathway for coordinating local activity of electrically nonexcitable cells, because identical patterns of ATP release were identified in human astrocytes, endothelial cells, and bronchial epithelial cells.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 99 (15), 9840-9845, 2002-07-03
Proceedings of the National Academy of Sciences
