書誌事項
- 公開日
- 1994-02
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1002/gcc.2870090206
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:p>The mutational spectrum for the <jats:italic>TP53</jats:italic> gene was investigated in a large series of bladder tumors and bladder tumor cell lines. Tumors and cell lines were screened for the presence of <jats:italic>TP53</jats:italic> point mutations by single‐strand conformational polymorphism analysis followed by direct sequencing. Mutations were detected in 16 of 88 (18%) tumors and 4 of 14 cell lines (28%). In total, twelve missense mutations, one nonsense mutation, three deletions, and two insertions were identified by direct sequencing. Of the thirteen point mutations sequenced, only one was a transition at a CpG site, whereas five G:C → T:A transversions were found, suggesting a major role for exogenous mutagens in bladder tumorigenesis. Tumors were also examined for loss of heterozygosity (LOH) on chromosome arm 17p. LOH of one or more markers on 17p was detected in 31 % of tumors. All eight tumors with a <jats:italic>TP53</jats:italic> mutation from patients informative at <jats:italic>TP53</jats:italic> had LOH, whereas nine tumors with LOH at <jats:italic>TP53</jats:italic> did not have an identified mutation. Three tumors had LOH on 17p at sites distal to the <jats:italic>TP53</jats:italic> locus but retained both <jats:italic>TP53</jats:italic> alleles, suggesting the involvement of another tumor suppressor gene on 17p in bladder tumorigenesis in some tumors. <jats:italic>Genes Chrom Cancer</jats:italic> 9:108‐118 (1994).© 1994 Wiley‐Liss, Inc.</jats:p>
収録刊行物
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- Genes, Chromosomes and Cancer
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Genes, Chromosomes and Cancer 9 (2), 108-118, 1994-02
Wiley