Higher Order Structure of DNA Controlled by the Redox State of Fe<sup>2+</sup>/Fe<sup>3+</sup>

DOI Web Site 被引用文献3件
  • Yuichi Yamasaki
    Contribution from the Graduate School of Human Informatics, Nagoya University, Nagoya 464-01, Japan
  • Kenichi Yoshikawa
    Contribution from the Graduate School of Human Informatics, Nagoya University, Nagoya 464-01, Japan

書誌事項

公開日
1997-11-01
DOI
  • 10.1021/ja971849r
公開者
American Chemical Society (ACS)

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説明

We performed fluorescence microscopic observation of the conformational change of individual T4DNAs (166 kbp) induced by Fe2+/Fe3+. Individual DNAs undergo a marked discrete transition from an elongated coil into a collapsed globule with an increase in the Fe3+ concentration at around 1−2 μM. On the other hand, DNAs remained in the elongated coil state with the addition of Fe2+ up to a concentration of 30 μM. Using these experimental results we tried to control the transition of DNA by the redox reaction of Fe2+/Fe3+. We found that collapsed globule DNA unfolds with the reduction of Fe3+. The results have been analyzed theoretically in terms of the double minima in the free energy profile of a single DNA molecule, indicating that the change in the translational entropy of the counterions is the main reason why high-valency ions are more effective in inducing the collapse.

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