Derivation of pluripotent stem cells from cultured human primordial germ cells

DOI Web Site 被引用文献44件
  • Michael J. Shamblott
    Departments of Gynecology and Obstetrics and Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21287; Kimmel Cancer Institute, Jefferson Medical College, Philadelphia, PA 19107; and Department of Gynecology and Obstetrics, Johns Hopkins Bayview Hospital, Baltimore, MD 21224
  • Joyce Axelman
    Departments of Gynecology and Obstetrics and Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21287; Kimmel Cancer Institute, Jefferson Medical College, Philadelphia, PA 19107; and Department of Gynecology and Obstetrics, Johns Hopkins Bayview Hospital, Baltimore, MD 21224
  • Shunping Wang
    Departments of Gynecology and Obstetrics and Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21287; Kimmel Cancer Institute, Jefferson Medical College, Philadelphia, PA 19107; and Department of Gynecology and Obstetrics, Johns Hopkins Bayview Hospital, Baltimore, MD 21224
  • Elizabeth M. Bugg
    Departments of Gynecology and Obstetrics and Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21287; Kimmel Cancer Institute, Jefferson Medical College, Philadelphia, PA 19107; and Department of Gynecology and Obstetrics, Johns Hopkins Bayview Hospital, Baltimore, MD 21224
  • John W. Littlefield
    Departments of Gynecology and Obstetrics and Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21287; Kimmel Cancer Institute, Jefferson Medical College, Philadelphia, PA 19107; and Department of Gynecology and Obstetrics, Johns Hopkins Bayview Hospital, Baltimore, MD 21224
  • Peter J. Donovan
    Departments of Gynecology and Obstetrics and Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21287; Kimmel Cancer Institute, Jefferson Medical College, Philadelphia, PA 19107; and Department of Gynecology and Obstetrics, Johns Hopkins Bayview Hospital, Baltimore, MD 21224
  • Paul D. Blumenthal
    Departments of Gynecology and Obstetrics and Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21287; Kimmel Cancer Institute, Jefferson Medical College, Philadelphia, PA 19107; and Department of Gynecology and Obstetrics, Johns Hopkins Bayview Hospital, Baltimore, MD 21224
  • George R. Huggins
    Departments of Gynecology and Obstetrics and Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21287; Kimmel Cancer Institute, Jefferson Medical College, Philadelphia, PA 19107; and Department of Gynecology and Obstetrics, Johns Hopkins Bayview Hospital, Baltimore, MD 21224
  • John D. Gearhart
    Departments of Gynecology and Obstetrics and Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21287; Kimmel Cancer Institute, Jefferson Medical College, Philadelphia, PA 19107; and Department of Gynecology and Obstetrics, Johns Hopkins Bayview Hospital, Baltimore, MD 21224

書誌事項

公開日
1998-11-10
DOI
  • 10.1073/pnas.95.23.13726
公開者
Proceedings of the National Academy of Sciences

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説明

<jats:p>Human pluripotent stem cells would be invaluable for<jats:italic>in vitro</jats:italic>studies of aspects of human embryogenesis. With the goal of establishing pluripotent stem cell lines, gonadal ridges and mesenteries containing primordial germ cells (PGCs, 5–9 weeks postfertilization) were cultured on mouse STO fibroblast feeder layers in the presence of human recombinant leukemia inhibitory factor, human recombinant basic fibroblast growth factor, and forskolin. Initially, single PGCs in culture were visualized by alkaline phosphatase activity staining. Over a period of 7–21 days, PGCs gave rise to large multicellular colonies resembling those of mouse pluripotent stem cells termed embryonic stem and embryonic germ (EG) cells. Throughout the culture period most cells within the colonies continued to be alkaline phosphatase-positive and tested positive against a panel of five immunological markers (SSEA-1, SSEA-3, SSEA-4, TRA-1–60, and TRA-1–81) that have been used routinely to characterize embryonic stem and EG cells. The cultured cells have been continuously passaged and found to be karyotypically normal and stable. Both XX and XY cell cultures have been obtained. Immunohistochemical analysis of embryoid bodies collected from these cultures revealed a wide variety of differentiated cell types, including derivatives of all three embryonic germ layers. Based on their origin and demonstrated properties, these human PGC-derived cultures meet the criteria for pluripotent stem cells and most closely resemble EG cells.</jats:p>

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