Nondestructive micropatterning of living animal cells using focused femtosecond laser-induced impulsive force

DOI PDF 被引用文献17件
  • Takahiro Kaji
    Osaka University Division of Frontier Materials Science, Graduate School of Engineering Science, , 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan
  • Syoji Ito
    Osaka University Division of Frontier Materials Science, Graduate School of Engineering Science, , 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan
  • Hiroshi Miyasaka
    Osaka University Division of Frontier Materials Science, Graduate School of Engineering Science, , 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan
  • Yoichiroh Hosokawa
    Osaka University Department of Applied Physics, , 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Venture Business Laboratory, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; and , 4–1–8, Honcho, Kawaguchi-Shi, Saitama 332–0012, Japan
  • Hiroshi Masuhara
    Osaka University Department of Applied Physics, , 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Venture Business Laboratory, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; and , 4–1–8, Honcho, Kawaguchi-Shi, Saitama 332–0012, Japan
  • Chisa Shukunami
    Kyoto University Department of Cell Differentiation, Institute for Frontier Medical Sciences, , 53 Kawahara-cho Shogoin, Sakyo-ku, Kyoto 606-8507, Japan and , 4–1–8, Honcho, Kawaguchi-Shi, Saitama 332–0012, Japan
  • Yuji Hiraki
    Kyoto University Department of Cell Differentiation, Institute for Frontier Medical Sciences, , 53 Kawahara-cho Shogoin, Sakyo-ku, Kyoto 606-8507, Japan and , 4–1–8, Honcho, Kawaguchi-Shi, Saitama 332–0012, Japan

書誌事項

公開日
2007-07-09
DOI
  • 10.1063/1.2753103
公開者
AIP Publishing

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説明

<jats:p>Micropatterning of mouse NIH3T3 fibroblast cells was performed using focused femtosecond laser-induced impulsive force in a culture medium. The cells were detached from an upper substrate by the force and transferred to an underlying substrate with less than spatial resolution of 80μm full width at half maximum. About 80% of the cells were confirmed to be alive at 3h after the patterning. The force exerted to the cell was investigated by high-speed imaging and estimated to be an order of micronewtons. The force origin was not only due to cavitation bubble but also due to shockwave and jet flow.</jats:p>

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