Use of a redox‐sensing GFP (c‐roGFP1) for real‐time monitoring of cytosol redox status in <i>Arabidopsis thaliana</i> water‐stressed plants
書誌事項
- 公開日
- 2010-01-15
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1016/j.febslet.2010.01.014
- 公開者
- Wiley
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説明
<jats:p>Using Arabidopsis plants transformed with a redox‐sensing green fluorescent protein targeted to the cytosol (c‐roGFP1), we have demonstrated, in real time, measurements of reversible changes of redox status in the cytosol of plants subjected to a gradual water‐stress, followed by re‐watering. Plants sensed water stress, and changed the redox potential of their cytosol to a more oxidized value after a gradually‐imposed water stress. Small variations in the cytosol redox potential and ascorbate (AA) values suggest that this parameter was tightly regulated. The re‐watering was paralleled by a return of water stress, redox potential and ascorbate to initial values, showing the reversibility of water stress and its consequences.</jats:p>
収録刊行物
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- FEBS Letters
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FEBS Letters 584 (5), 889-897, 2010-01-15
Wiley