Activity of H(+)-ATPase in ruminal bacteria with special reference to acid tolerance

  • T Miwa
    Department of Agriculture, Meiji University, Kawasaki, Japan.
  • H Esaki
    Department of Agriculture, Meiji University, Kawasaki, Japan.
  • J Umemori
    Department of Agriculture, Meiji University, Kawasaki, Japan.
  • T Hino
    Department of Agriculture, Meiji University, Kawasaki, Japan.

書誌事項

公開日
1997-06
権利情報
  • https://journals.asm.org/non-commercial-tdm-license
DOI
  • 10.1128/aem.63.6.2155-2158.1997
公開者
American Society for Microbiology

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説明

<jats:p>Batch culture experiments showed that permeabilized cells and membranes of Ruminococcus albus and Fibrobacter succinogenes, acid-intolerant celluloytic bacteria, have only one-fourth to one-fifth as much H(+)-ATPase as Megasphaera elsdenii and Streptococcus bovis, which are relatively acid tolerant. Even in the cells grown in continuous culture at pH 7.0, the acid-intolerant bacteria contained less than half as much H(+)-ATPase as the acid-tolerant bacteria. The amounts of H(+)-ATPase in the acid-tolerant bacteria were increased by more than twofold when the cells were grown at the lowest pH permitting growth, whereas little increase was observed in the case of the acid-intolerant bacteria. These results indicate that the acid-intolerant bacteria not only contain smaller amounts of H(+)-ATPase at neutral pH but also have a lower capacity to enhance the level of H(+)-ATPase in response to low pH than the acid-tolerant bacteria. In addition, the H(+)-ATPases of the acid-intolerant bacteria were more sensitive to low pH than those of the acid-tolerant bacteria, although the optimal pHs were similar.</jats:p>

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