Monitoring Genetic stability in<i>Quercus serrata</i>Thunb. Somatic Embryogenesis Using RAPD Markers

DOI Web Site 被引用文献1件
  • Ramesh C. Thakur
    Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687, Japan.
  • Susumu Goto
    Fukuoka Prefecture Forest Research and Extension Center, Kurume, Fukuoka 839-0827, Japan.
  • Katsuaki Ishii
    Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687, Japan.
  • S. Mohan Jain
    University of Helsinki, 00014-Finland.

書誌事項

公開日
1999-05
DOI
  • 10.1007/bf02762241
公開者
Informa UK Limited

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説明

Genetic stability of propagules regeneratedvia somatic embryogenesis is of paramount importance for its application to clonal forestry. Random amplified polymorphic DNA (RAPD) markers were used to determine the genetic stability in somatic embryogenesis ofQuercus serrata Thunb. (Japanese white oak). Forty samples from an embryogenic line, consisting of regenerated plantlets, somatic embryos, and embryogenic calli, were examined using 54 decanucleotide primers. A total of 6520 clear reproducible bands obtained from these studies exhibited no aberration in RAPD banding pattern among the tested samples. Our results show that somaclonal variation is absent in our plant propagation system. The genetic stability is discussed in terms of the origin of somatic embryos.

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