Kinetics of inhibition of methane oxidation by nitrate, nitrite, and ammonium in a humisol
書誌事項
- 公開日
- 1995-08
- 権利情報
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- https://journals.asm.org/non-commercial-tdm-license
- DOI
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- 10.1128/aem.61.8.3129-3135.1995
- 公開者
- American Society for Microbiology
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説明
<jats:p>The kinetics of inhibition of CH(inf4) oxidation by NH(inf4)(sup+), NO(inf2)(sup-), and NO(inf3)(sup-) in a humisol was investigated. Soil slurries exhibited nearly standard Michaelis-Menten kinetics, with half-saturation constant [K(infm(app))] values for CH(inf4) of 50 to 200 parts per million of volume (ppmv) and V(infmax) values of 1.1 to 2.5 nmol of CH(inf4) g of dry soil(sup-1) h(sup-1). With one soil sample, NH(inf4)(sup+) acted as a simple competitive inhibitor, with an estimated K(infi) of 8 (mu)M NH(inf4)(sup+) (18 nM NH(inf3)). With another soil sample, the response to NH(inf4)(sup+) addition was more complex and the inhibitory effect of NH(inf4)(sup+) was greater than predicted by a simple competitive model at low CH(inf4) concentrations (<50 ppmv). This was probably due to NO(inf2)(sup-) produced through NH(inf4)(sup+) oxidation. Added NO(inf2)(sup-) was inherently more inhibitory of CH(inf4) oxidation at low CH(inf4) concentrations, and more NO(inf2)(sup-) was produced as the CH(inf4)-to-NH(inf4)(sup+) ratio decreased and the competitive balance shifted. NaNO(inf3) was a noncompetitive inhibitor of CH(inf4) oxidation, but inhibition was evident only at >10 mM concentrations, which also altered soil pHs. Similar concentrations of NaCl were also inhibitory of CH(inf4) oxidation, so there may be no special inhibitory mechanism of nitrate per se.</jats:p>
収録刊行物
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- Applied and Environmental Microbiology
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Applied and Environmental Microbiology 61 (8), 3129-3135, 1995-08
American Society for Microbiology