Structural hierarchy of several proteins observed by wide-angle solution scattering

書誌事項

公開日
2002-06-30
権利情報
  • http://journals.iucr.org/services/copyrightpolicy.html
  • http://journals.iucr.org/services/copyrightpolicy.html#TDM
DOI
  • 10.1107/s0909049502006593
公開者
International Union of Crystallography (IUCr)

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説明

In the present study using a high-intensity X-ray beam from a third-generation synchrotron radiation source, it is demonstrated that a wide-angle X-ray scattering (WAXS) profile from several globular proteins in solution can reflect not only the overall structures (approximately 300 A distance resolution) but also intramolecular structures ranging to secondary structures (approximately 2.5 A distance resolution). The proteins treated in the present experiments are classified as different types of structure categories, namely, as all-alpha, all-beta and alpha + beta proteins. Here the full-range experimental scattering curves are compared with the theoretical curves, suggesting a further availability of the SR-WAXS method for studies of structure hierarchy and the function of proteins in solutions.

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