The transcriptome of early chicken embryos reveals signaling pathways governing rapid asymmetric cellularization and lineage segregation
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- Young Sun Hwang
- Research Institute of Agriculture and Life Sciences, Seoul National University 1 Department of Agricultural Biotechnology and , Seoul 08826 , Korea
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- Minseok Seo
- CHO&KIM Genomics, SNU Research Park, Seoul National University Mt.4-2 2 , Seoul 08826 , Korea
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- Bo Ram Lee
- Research Institute of Agriculture and Life Sciences, Seoul National University 1 Department of Agricultural Biotechnology and , Seoul 08826 , Korea
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- Hong Jo Lee
- Research Institute of Agriculture and Life Sciences, Seoul National University 1 Department of Agricultural Biotechnology and , Seoul 08826 , Korea
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- Young Hyun Park
- Research Institute of Agriculture and Life Sciences, Seoul National University 1 Department of Agricultural Biotechnology and , Seoul 08826 , Korea
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- Sang Kyung Kim
- Research Institute of Agriculture and Life Sciences, Seoul National University 1 Department of Agricultural Biotechnology and , Seoul 08826 , Korea
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- Hyung Chul Lee
- Research Institute of Agriculture and Life Sciences, Seoul National University 1 Department of Agricultural Biotechnology and , Seoul 08826 , Korea
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- Hee Jung Choi
- Research Institute of Agriculture and Life Sciences, Seoul National University 1 Department of Agricultural Biotechnology and , Seoul 08826 , Korea
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- Joon Yoon
- Interdisciplinary Program in Bioinformatics, Seoul National University 5 , Seoul 08826 , Korea
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- Heebal Kim
- Research Institute of Agriculture and Life Sciences, Seoul National University 1 Department of Agricultural Biotechnology and , Seoul 08826 , Korea
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- Jae Yong Han
- Research Institute of Agriculture and Life Sciences, Seoul National University 1 Department of Agricultural Biotechnology and , Seoul 08826 , Korea
説明
<jats:p>The phylogenomics and comparative functional genomics of avian species were investigated in the Bird 10,000 Genomes (B10K) project because of the important evolutionary position of birds and their value as a research model. However, the systematic profiling of transcriptional changes prior to oviposition has not been investigated in avian species because of the practical difficulties in obtaining pre-oviposited eggs. In this study, a total of 137 pre-oviposited embryos were collected from hen ovaries and oviducts and subjected to RNA-sequencing analyses. Two waves of chicken zygotic genome activation (ZGA) were observed. Functionally distinct developmental programs involving Notch, MAPK, Wnt and TGFβ signaling were separately detected during cleavage and area pellucida formation. Furthermore, the early stages of chicken development were compared with the human and mouse counterparts, highlighting chicken-specific signaling pathways and gradually analogous gene expression via ZGA. These findings provide a genome-wide understanding of avian embryogenesis and comparisons among amniotes.</jats:p>
収録刊行物
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- Development
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Development 145 (6), dev157453-, 2018-03-14
The Company of Biologists
