Automated analysis of hexosamines by high-performance liquid chromatography with photometric and fluorimetric postcolumn labeling using 2-cyanoacetamide
書誌事項
- 公開日
- 1983-10
- 権利情報
-
- https://www.elsevier.com/tdm/userlicense/1.0/
- https://www.elsevier.com/legal/tdmrep-license
- DOI
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- 10.1016/0003-2697(83)90327-5
- 公開者
- Elsevier BV
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説明
Glucosamine and galactosamine were well separated in ca. 60 min on a Hitachi 2617 column (polystyrene sulfonate type, 4 mm X 25 cm) with a borate buffer (pH 7.5) containing sodium chloride. The hexosamines in the eluate were monitored fluorimetrically and photometrically at 331 (excitation)/383 (emission) and 276 nm, respectively, by postcolumn labeling with 2-cyanoacetamide. This simple method allowed the simultaneous, automated determination of 10-500 nmol of glucosamine and galactosamine with high reproducibility. This method was applied successfully to the analysis of hexosamines in some glycoconjugates.
収録刊行物
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- Analytical Biochemistry
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Analytical Biochemistry 134 (2), 483-488, 1983-10
Elsevier BV