A new gene required for cellulose production and a gene encoding cellulolytic activity in Acetobacter xylinum are colocalized with the bcs operon

DOI Web Site 被引用文献20件
  • R Standal
    UNIGEN Center for Molecular Biology, University of Trondheim, Norway.
  • T G Iversen
    UNIGEN Center for Molecular Biology, University of Trondheim, Norway.
  • D H Coucheron
    UNIGEN Center for Molecular Biology, University of Trondheim, Norway.
  • E Fjaervik
    UNIGEN Center for Molecular Biology, University of Trondheim, Norway.
  • J M Blatny
    UNIGEN Center for Molecular Biology, University of Trondheim, Norway.
  • S Valla
    UNIGEN Center for Molecular Biology, University of Trondheim, Norway.

書誌事項

公開日
1994-02
権利情報
  • https://journals.asm.org/non-commercial-tdm-license
DOI
  • 10.1128/jb.176.3.665-672.1994
公開者
American Society for Microbiology

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説明

<jats:p>Recently, it was shown that a cellulose-negative mutant (Cel1) of Acetobacter xylinum ATCC 23769 carried an insertion of an indigenous transposable element (IS1031A) about 500 bp upstream of the bcs operon, required for cellulose synthesis. Here we show that Cel1 can be complemented by wild-type DNA covering the insertion point. Nucleotide sequencing of this region revealed the presence of two open reading frames, ORF1 and ORF2. ORF2, which is disrupted by the IS1031A insertion in Cel1, potentially encodes the complementing function. ORF1 encodes a protein (CMCax) with significant homology to previously described endoglucanases. A cloned DNA fragment containing ORF1 expressed a carboxymethyl cellulose-hydrolyzing activity in Escherichia coli. In A. xylinum, CMCax is secreted into the culture growth medium. The CMCax mature protein consists of 322 amino acids and has a molecular mass of 35.6 kDa.</jats:p>

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