NBD‐Based Green Fluorescent Ligands for Typing of Thymine‐Related SNPs by Using an Abasic Site‐Containing Probe DNA
書誌事項
- 公開日
- 2009-12-23
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1002/cbic.200900530
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:p><jats:italic>The binding behavior of green fluorescent ligands, derivatives of 7‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD), with DNA duplexes containing an abasic (AP) site is studied by thermal denaturation and fluorescence experiments. Among NBD derivatives,</jats:italic> N<jats:sup>1</jats:sup><jats:italic>‐(7‐nitrobenzo[</jats:italic>c<jats:italic>][1,2,5]oxadiazol‐4‐yl)propane‐1,3‐diamine (NBD‐NH<jats:sub>2</jats:sub>) is found to bind selectively to the thymine base opposite an AP site in a DNA duplex with a binding affinity of 1.52×10<jats:sup>6</jats:sup> <jats:sc>M</jats:sc><jats:sup>−1</jats:sup>. From molecular modeling studies, it is suggested that the NBD moiety binds to thymine at the AP site and a protonated amino group tethered to the NBD moiety interacts with the guanine base flanking the AP site. Green fluorescent NBD‐NH<jats:sub>2</jats:sub> is successfully applied for simultaneous G>T genotyping of PCR amplification products in a single cuvette in combination with a blue fluorescent ligand, 2‐amino‐6,7‐dimethyl‐4‐hydroxypteridine (diMe‐pteridine).</jats:italic></jats:p>
収録刊行物
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- ChemBioChem
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ChemBioChem 11 (1), 94-100, 2009-12-23
Wiley