Fluorescence Excitation Emission Matrices of Human Tissue: A System for <i>in vivo</i> Measurement and Method of Data Analysis
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- Andrés F. Zuluaga
- Department of Electrical and Computer Engineering, The University of Texas at Austin, Austin, Texas 78712 (A.F.Z., U.U., H.F., R.R.-K.); Candela Corporation, Wayland, Massachusetts (A.D.); and Department of Head and Neck Surgery, The UT MD Anderson Cancer Center, Houston, Texas 77030 (A.G., R.J., B.K.)
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- Urs Utzinger
- Department of Electrical and Computer Engineering, The University of Texas at Austin, Austin, Texas 78712 (A.F.Z., U.U., H.F., R.R.-K.); Candela Corporation, Wayland, Massachusetts (A.D.); and Department of Head and Neck Surgery, The UT MD Anderson Cancer Center, Houston, Texas 77030 (A.G., R.J., B.K.)
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- Anthony Durkin
- Department of Electrical and Computer Engineering, The University of Texas at Austin, Austin, Texas 78712 (A.F.Z., U.U., H.F., R.R.-K.); Candela Corporation, Wayland, Massachusetts (A.D.); and Department of Head and Neck Surgery, The UT MD Anderson Cancer Center, Houston, Texas 77030 (A.G., R.J., B.K.)
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- Holger Fuchs
- Department of Electrical and Computer Engineering, The University of Texas at Austin, Austin, Texas 78712 (A.F.Z., U.U., H.F., R.R.-K.); Candela Corporation, Wayland, Massachusetts (A.D.); and Department of Head and Neck Surgery, The UT MD Anderson Cancer Center, Houston, Texas 77030 (A.G., R.J., B.K.)
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- Ann Gillenwater
- Department of Electrical and Computer Engineering, The University of Texas at Austin, Austin, Texas 78712 (A.F.Z., U.U., H.F., R.R.-K.); Candela Corporation, Wayland, Massachusetts (A.D.); and Department of Head and Neck Surgery, The UT MD Anderson Cancer Center, Houston, Texas 77030 (A.G., R.J., B.K.)
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- Rhonda Jacob
- Department of Electrical and Computer Engineering, The University of Texas at Austin, Austin, Texas 78712 (A.F.Z., U.U., H.F., R.R.-K.); Candela Corporation, Wayland, Massachusetts (A.D.); and Department of Head and Neck Surgery, The UT MD Anderson Cancer Center, Houston, Texas 77030 (A.G., R.J., B.K.)
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- Bonnie Kemp
- Department of Electrical and Computer Engineering, The University of Texas at Austin, Austin, Texas 78712 (A.F.Z., U.U., H.F., R.R.-K.); Candela Corporation, Wayland, Massachusetts (A.D.); and Department of Head and Neck Surgery, The UT MD Anderson Cancer Center, Houston, Texas 77030 (A.G., R.J., B.K.)
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- James Fan
- Department of Electrical and Computer Engineering, The University of Texas at Austin, Austin, Texas 78712 (A.F.Z., U.U., H.F., R.R.-K.); Candela Corporation, Wayland, Massachusetts (A.D.); and Department of Head and Neck Surgery, The UT MD Anderson Cancer Center, Houston, Texas 77030 (A.G., R.J., B.K.)
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- Rebecca Richards-Kortum
- Department of Electrical and Computer Engineering, The University of Texas at Austin, Austin, Texas 78712 (A.F.Z., U.U., H.F., R.R.-K.); Candela Corporation, Wayland, Massachusetts (A.D.); and Department of Head and Neck Surgery, The UT MD Anderson Cancer Center, Houston, Texas 77030 (A.G., R.J., B.K.)
抄録
<jats:p> We describe a system capable of measuring spatially resolved reflectance spectra from 380 to 950 nm and fluorescence excitation emission matrices from 330 to 500 nm excitation and 380 to 700 nm emission in vivo. System performance was compared to that of a standard scanning spectrofluorimeter. This “FastEEM” system was used to interrogate human normal and neoplastic oral cavity mucosa in vivo. Measurements were made through a fiber-optic probe and require 4 min total measurement time. We present a method based on autocorrelation vectors to identify excitation and emission wavelengths where the spectra of normal and pathologic tissues differ most. The FastEEM system provides a tool with which to study the relative diagnostic ability of changes in absorption, scattering, and fluorescence properties of tissue. </jats:p>
収録刊行物
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- Applied Spectroscopy
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Applied Spectroscopy 53 (3), 302-311, 1999-03
SAGE Publications