Identification of perivascular mesenchymal stromal/stem cells by flow cytometry

DOI Web Site Web Site 被引用文献2件
  • Mirko Corselli
    UCLA—Orthopaedic Hospital Department of Orthopaedic Surgery The Orthopaedic Hospital Research Center Los Angeles California
  • Mihaela Crisan
    Department of Cell Biology Erasmus MC Stem Cell Institute Rotterdam The Netherlands
  • Iain R. Murray
    Centre for Cardiovascular Science and Centre for Regenerative Medicine University of Edinburgh Edinburgh United Kingdom
  • Christopher C. West
    Centre for Cardiovascular Science and Centre for Regenerative Medicine University of Edinburgh Edinburgh United Kingdom
  • Jessica Scholes
    Eli and Edythe Broad Stem Cell Research Center Flow Cytometry Core, University of California Los Angeles California
  • Felicia Codrea
    Eli and Edythe Broad Stem Cell Research Center Flow Cytometry Core, University of California Los Angeles California
  • Nusrat Khan
    Centre for Cardiovascular Science and Centre for Regenerative Medicine University of Edinburgh Edinburgh United Kingdom
  • Bruno Péault
    UCLA—Orthopaedic Hospital Department of Orthopaedic Surgery The Orthopaedic Hospital Research Center Los Angeles California

書誌事項

公開日
2013-08
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1002/cyto.a.22313
公開者
Wiley

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説明

<jats:p>Mesenchymal stem/stromal cells (MSCs) are adult multipotent progenitors of great promise for cell therapy. MSCs can mediate tissue regeneration, immunomodulation, and hematopoiesis support. Despite the unique properties of MSCs and their broad range of potential clinical applications, the very nature of these cells has been uncertain. Furthermore, MSCs are heterogeneous and only defined subpopulations of these are endowed with the particular abilities to sustain hematopoietic stem cells, regulate immune responses, or differentiate into mesodermal cell lineages. It is becoming evident that current criteria used to define cultured polyclonal MSCs (expression of nonspecific markers and in vitro mesodermal differentiation) are not sufficient to fully understand and exploit the potential of these cells. Here, we describe how flow cytometry has been used to reveal a perivascular origin of MSCs. As a result, the prospective purification of MSCs and specialized subsets thereof is now possible, and the clinical use of purified autologous MSCs is now within reach. © 2013 International Society for Advancement of Cytometry</jats:p>

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