ToF‐SIMS analysis of biomolecules in the model organism <i>Caenorhabditis elegans</i>

DOI Web Site Web Site 被引用文献1件
  • Florian M. Geier
    Biomolecular Medicine, Department of Surgery and Cancer, Faculty of Medicine, South Kensington Campus Imperial College London London SW7 2AZ UK
  • Sarah Fearn
    Department of Material Sciences, Faculty of Engineering, South Kensington Campus Imperial College London London SW7 2AZ UK
  • Jacob G. Bundy
    Biomolecular Medicine, Department of Surgery and Cancer, Faculty of Medicine, South Kensington Campus Imperial College London London SW7 2AZ UK
  • David S. McPhail
    Department of Material Sciences, Faculty of Engineering, South Kensington Campus Imperial College London London SW7 2AZ UK

書誌事項

公開日
2012-07-17
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1002/sia.5110
公開者
Wiley

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説明

<jats:p> <jats:italic>C.elegans</jats:italic> is a biomedical key model organism as it is a simple, easy to maintain eukaryote, which shares many gene homologues with higher mammals. As each of its cells has been traced during development and characterized by light microscopy, mass spectrometry‐based time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS) imaging seems to promise exciting new insights. In this study, we have investigated simple but basic factors for ToF‐SIMS‐based imaging of <jats:italic>C.elegans</jats:italic> . By comparing chemical standards and two authentic <jats:italic>C. elegans</jats:italic> mutant extracts (N2 and Daf‐2), we found that for our purposes, Bi <jats:sub>3</jats:sub> <jats:sup>+</jats:sup> is better suited due to its higher mass and spatial resolution. We also investigated the use of light microscopy slides as imaging substrates and gold coating as standard for mass calibration in higher mass ranges. Our findings and preliminary imaging results show that ToF‐SIMS is a well‐suited platform for mass spectrometry imaging. Copyright © 2012 John Wiley & Sons, Ltd. </jats:p>

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