Three-dimensional manipulation of single cells using surface acoustic waves

  • Feng Guo
    Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802;
  • Zhangming Mao
    Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802;
  • Yuchao Chen
    Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802;
  • Zhiwei Xie
    Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA 16802;
  • James P. Lata
    Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802;
  • Peng Li
    Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802;
  • Liqiang Ren
    Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802;
  • Jiayang Liu
    Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802;
  • Jian Yang
    Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA 16802;
  • Ming Dao
    Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139;
  • Subra Suresh
    Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213;
  • Tony Jun Huang
    Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802;

書誌事項

公開日
2016-01-25
権利情報
  • http://www.pnas.org/preview_site/misc/userlicense.xhtml
DOI
  • 10.1073/pnas.1524813113
公開者
Proceedings of the National Academy of Sciences

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説明

<jats:title>Significance</jats:title> <jats:p>We present 3D acoustic tweezers, which can trap and manipulate single cells and particles along three mutually orthogonal axes of motion by recourse to surface acoustic waves. We use 3D acoustic tweezers to pick up single cells, or entire cell assemblies, and deliver them to desired locations to create 2D and 3D cell patterns, or print the cells into complex shapes. This technology is thus shown to offer better performance over prior cell manipulation techniques in terms of both accurate and precise motion in a noninvasive, label-free, and contactless manner. This method offers the potential to accurately print 3D multicellular architectures for applications in biomanufacturing, tissue engineering, regenerative medicine, neuroscience, and cancer metastasis research.</jats:p>

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