FT‐IR study of the Ca<sup>2+</sup>‐binding to bovine α‐lactalbumin

書誌事項

タイトル別名
  • Relationships between the type of coordination and characteristics of the bands due to the Asp COO<sup>−</sup> groups in the Ca<sup>2+</sup>‐binding site
公開日
1997-11-03
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1016/s0014-5793(97)01274-x
公開者
Wiley

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説明

<jats:p>Fourier‐transform infrared spectroscopy (FT‐IR) was applied to examine relationships between the type of coordination and the COO<jats:sup>−</jats:sup> antisymmetric and symmetric stretches of the COO<jats:sup>−</jats:sup> groups in the Ca<jats:sup>2+</jats:sup>‐binding site of bovine α‐lactalbumin. The peaks at 1593, 1578, 1425, and 1403 cm<jats:sup>−1</jats:sup> were assigned to the COO<jats:sup>−</jats:sup> groups of Asp‐82, 87, and 88 coordinating to Ca<jats:sup>2+</jats:sup> in the pseudo bridging mode, according to the results of X‐ray crystallography. The bands due to the COO<jats:sup>−</jats:sup> groups were quite similar to each other between α‐lactalbumin and EDTA which is the model compound for the pseudo bridging state.</jats:p>

収録刊行物

  • FEBS Letters

    FEBS Letters 417 (1), 153-156, 1997-11-03

    Wiley

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