書誌事項
- 公開日
- 1981-02-01
- 権利情報
-
- https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model
- DOI
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- 10.1093/clinchem/27.2.286
- 公開者
- Oxford University Press (OUP)
この論文をさがす
説明
<jats:title>Abstract</jats:title> <jats:p>We describe simple, precise methods, based on second and fourth-derivative spectroscopy, of analysis for paraquat in serum, plasma, or dialysis fluid. Plasma paraquat, after ion-pair extraction and dithionite reduction, yields a sharp, stable absorption peak at 396 nm. Derivative spectroscopy confers enhanced sensitivity (detection limit, 5 microgram/L) and improved rejection of spectroscopic interference by diquat, hemolysis, bilirubin, or lipemia. Derivative peak amplitudes are linearly related to paraquat dichloride concentration from 10 to 1000 microgram/L in plasma, and the methods are precise (CV 2.7-5.2% for 50 microgram/L concentration) and accurate. Results of derivative assays for 18 cases of paraquat poisoning correlate well with those by radioimmunoassay (r = 0.999). Derivative spectroscopy enables monitoring of paraquat concentrations in the plasma of poisoned patients during treatment by hemoperfusion or hemodialysis. A rapid assay developed for dialysis fluid (detection limit, 2 microgram/L) permits the efficacy of hemodialysis to be routinely assessed.</jats:p>
収録刊行物
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- Clinical Chemistry
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Clinical Chemistry 27 (2), 286-292, 1981-02-01
Oxford University Press (OUP)