Mutation of Host <i>dnaJ</i> Homolog Inhibits Brome Mosaic Virus Negative-Strand RNA Synthesis
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- Yuriko Tomita
- Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589
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- Tomomitsu Mizuno
- Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589
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- Juana Díez
- Institute for Molecular Virology
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- Satoshi Naito
- Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589
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- Paul Ahlquist
- Institute for Molecular Virology
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- Masayuki Ishikawa
- Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589
書誌事項
- 公開日
- 2003-03
- 権利情報
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- https://journals.asm.org/non-commercial-tdm-license
- DOI
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- 10.1128/jvi.77.5.2990-2997.2003
- 公開者
- American Society for Microbiology
この論文をさがす
説明
<jats:title>ABSTRACT</jats:title> <jats:p> The replication of positive-strand RNA viruses involves not only viral proteins but also multiple cellular proteins and intracellular membranes. In both plant cells and the yeast <jats:italic>Saccharomyces cerevisiae</jats:italic> , brome mosaic virus (BMV), a member of the alphavirus-like superfamily, replicates its RNA in endoplasmic reticulum (ER)-associated complexes containing viral 1a and 2a proteins. Prior to negative-strand RNA synthesis, 1a localizes to ER membranes and recruits both positive-strand BMV RNA templates and the polymerase-like 2a protein to ER membranes. Here, we show that BMV RNA replication in <jats:italic>S. cerevisiae</jats:italic> is markedly inhibited by a mutation in the host <jats:italic>YDJ1</jats:italic> gene, which encodes a chaperone Ydj1p related to <jats:italic>Escherichia coli</jats:italic> DnaJ. In the <jats:italic>ydj1</jats:italic> mutant, negative-strand RNA accumulation was inhibited even though 1a protein associated with membranes and the positive-strand RNA3 replication template and 2a protein were recruited to membranes as in wild-type cells. In addition, we found that in <jats:italic>ydj1</jats:italic> mutant cells but not wild-type cells, a fraction of 2a protein accumulated in a membrane-free but insoluble, rapidly sedimenting form. These and other results show that Ydj1p is involved in forming BMV replication complexes active in negative-strand RNA synthesis and suggest that a chaperone system involving Ydj1p participates in 2a protein folding or assembly into the active replication complex. </jats:p>
収録刊行物
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- Journal of Virology
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Journal of Virology 77 (5), 2990-2997, 2003-03
American Society for Microbiology