B cell subpopulations separated by CD27 and crucial collaboration of CD27<sup>+</sup> B cells and helper T cells in immunoglobulin production
書誌事項
- 公開日
- 1997-08
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1002/eji.1830270835
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Abstract</jats:title><jats:p>B cell immunoglobulin production is regulated by helper T cells through direct interaction and secreted cytokines. In the present study, we functionally analyzed CD27 in cord and peripheral blood B cells. Adult peripheral blood B cells were separated into CD27<jats:sup>+</jats:sup> and CD27<jats:sup>−</jats:sup> cells, which differed in their morphology. Cord blood B cells did not express CD27, and CD27 expression on peripheral blood B cells increased with age. Only CD27<jats:sup>+</jats:sup> B cells had the ability to produce immunoglobulin, which was increased by contact with a tumor necrosis factor‐related transmembrane ligand, CD70. Adult peripheral blood CD27<jats:sup>+</jats:sup> B cells can be further subdivided into two discrete subtypes: IgD<jats:sup>−</jats:sup>CD27<jats:sup>+</jats:sup> and IgD<jats:sup>+</jats:sup> CD27<jats:sup>+</jats:sup> B cells. IgD<jats:sup>−</jats:sup> CD27<jats:sup>+</jats:sup> B cells produce IgG, IgM and IgA, whereas IgD<jats:sup>+</jats:sup> CD27<jats:sup>+</jats:sup> B cells predominantly produce IgM. The addition of activated CD4<jats:sup>+</jats:sup> CD45RO T cells expressing CD70 caused down‐regulation of CD27 expression on activated B cells, and this down‐modulation was completely blocked by anti‐CD70 monoclonal antibody, indicating direct T‐B cell contact via CD27/CD70. The triggering via CD27 and CD40 additively increased the immunoglobulin production under <jats:italic>Staphylococcus aureus</jats:italic> Cowan strain plus interleukin‐2 stimulation. Taken together, our findings demonstrate that peripheral blood B cells are separated into subpopulations by CD27 and IgD expression and that CD27<jats:sup>+</jats:sup> B cells produce large amounts of immunoglobulin by interaction with the CD70 molecule.</jats:p>
収録刊行物
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- European Journal of Immunology
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European Journal of Immunology 27 (8), 2073-2079, 1997-08
Wiley