Characterization of a <i>Saccharomyces cerevisiae</i> NADP(H)‐dependent alcohol dehydrogenase (ADHVII), a member of the cinnamyl alcohol dehydrogenase family
書誌事項
- 公開日
- 2002-10-30
- 権利情報
-
- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
-
- 10.1046/j.1432-1033.2002.03296.x
- 公開者
- Wiley
この論文をさがす
説明
<jats:p>A new NADP(H)‐dependent alcohol dehydrogenase (the <jats:italic>YCR105W</jats:italic> gene product, ADHVII) has been identified in <jats:italic>Saccharomyces cerevisiae</jats:italic>. The enzyme has been purified to homogeneity and found to be a homodimer of 40 kDa subunits and a pI of 6.2–6.4. ADHVII shows a broad substrate specificity similar to the recently characterized ADHVI (64% identity), although they show some differences in kinetic properties. ADHVI and ADHVII are the only members of the cinnamyl alcohol dehydrogenase family in yeast. Simultaneous deletion of <jats:italic>ADH6</jats:italic> and <jats:italic>ADH7</jats:italic> was not lethal for the yeast. Both enzymes could participate in the synthesis of fusel alcohols, ligninolysis and NADP(H) homeostasis.</jats:p>
収録刊行物
-
- European Journal of Biochemistry
-
European Journal of Biochemistry 269 (22), 5738-5745, 2002-10-30
Wiley