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- Somasekar Seshagiri
- Departments of Entomology and Genetics, The University of Georgia, Athens, GA 30602
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- Lois K. Miller
- Departments of Entomology and Genetics, The University of Georgia, Athens, GA 30602
書誌事項
- 公開日
- 1997-12-09
- DOI
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- 10.1073/pnas.94.25.13606
- 公開者
- Proceedings of the National Academy of Sciences
この論文をさがす
説明
<jats:p> We have investigated the ability of Sf-caspase-1 and two mammalian caspases, caspase-1 and caspase-3, to induce apoptosis in <jats:italic>Spodoptera frugiperda</jats:italic> Sf-21 insect cells. While the transient expression of the pro-Sf-caspase-1 did not induce apoptosis, expression of the pro-domain deleted form, p31, or coexpression of the two subunits of mature Sf-caspase-1, p19 and p12, induced apoptosis in Sf-21 cells. The behavior of Sf-caspase-1 resembled that of the closely related mammalian caspase, caspase-3, and contrasted with that of the mammalian caspase-1, the pro-form of which was active in inducing apoptosis in Sf-21 cells. The baculovirus caspase inhibitor P35 blocked apoptosis induced by active forms of all three caspases. In contrast, members of the baculovirus inhibitor of apoptosis (IAP) family failed to block active caspase-induced apoptosis. However, during viral infection, expression of OpIAP or CpIAP blocked the activation of pro-Sf-caspase-1 and the associated induction of apoptosis. Thus, the mechanism by which baculovirus IAPs inhibit apoptosis is distinct from the mechanism by which P35 blocks apoptosis and involves inhibition of the activation of pro-caspases like Sf-caspase-1. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 94 (25), 13606-13611, 1997-12-09
Proceedings of the National Academy of Sciences
