The role of Vδ2-negative γδ T cells during cytomegalovirus reactivation in recipients of allogeneic stem cell transplantation
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- Andrea Knight
- Anthony Nolan Research Institute, London, United Kingdom; and
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- Alejandro J. Madrigal
- Anthony Nolan Research Institute, London, United Kingdom; and
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- Sarah Grace
- Department of Haematology, University College London, London, United Kingdom
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- Janani Sivakumaran
- Department of Haematology, University College London, London, United Kingdom
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- Panagiotis Kottaridis
- Department of Haematology, University College London, London, United Kingdom
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- Stephen Mackinnon
- Department of Haematology, University College London, London, United Kingdom
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- Paul J. Travers
- Anthony Nolan Research Institute, London, United Kingdom; and
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- Mark W. Lowdell
- Department of Haematology, University College London, London, United Kingdom
説明
<jats:title>Abstract</jats:title><jats:p>Reactivation of cytomegalovirus (CMV) remains a serious complication after allogeneic stem cell transplantation, but the role of γδ T cells is undefined. We have studied the immune reconstitution of Vδ2negative (Vδ2neg) γδ T cells, including Vδ1 and Vδ3 subsets and Vδ2positive (Vδ2pos) γδ T cells in 40 patients during the first 24 months after stem cell transplantation. Significant long-term expansions of Vδ2neg but not Vδ2pos γδ T cells were observed during CMV reactivation early after transplantation, suggesting direct involvement of γδ T cells in anti-CMV immune responses. Similarly, significantly higher numbers of Vδ2neg γδ T cells were detected in CMV-seropositive healthy persons compared with seronegative donors; the absolute numbers of Vδ2pos cells were not significantly different. The expansion of Vδ2neg γδ T cells appeared to be CMV-related because it was absent in CMV-negative/Epstein-Barr virus-positive patients. T-cell receptor-δ chain determining region 3 spectratyping of Vδ2neg γδ T cells in healthy subjects and patients showed restricted clonality. Polyclonal Vδ2neg cell lines generated from CMV-seropositive healthy donors and from a recipient of a graft from a CMV-positive donor lysed CMV-infected targets in all cases. Our study shows new evidence for role of γδ T cells in the immune response to CMV reactivation in transplantation recipients.</jats:p>
収録刊行物
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- Blood
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Blood 116 (12), 2164-2172, 2010-09-23
American Society of Hematology