Pig Cloning by Microinjection of Fetal Fibroblast Nuclei
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- Akira Onishi
- Department of Animal Breeding and Genetics, National Institute of Animal Industry, Tsukuba Norin Danchi, Ibaraki-ken 305–0901, Japan.
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- Masaki Iwamoto
- Basic Research Division, Prima Meatpackers, Tsuchiura-shi, Ibaraki-ken 300–0841, Japan.
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- Tomiji Akita
- Department of Animal Breeding and Genetics, National Institute of Animal Industry, Tsukuba Norin Danchi, Ibaraki-ken 305–0901, Japan.
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- Satoshi Mikawa
- Department of Animal Breeding and Genetics, National Institute of Animal Industry, Tsukuba Norin Danchi, Ibaraki-ken 305–0901, Japan.
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- Kumiko Takeda
- Department of Animal Breeding and Genetics, National Institute of Animal Industry, Tsukuba Norin Danchi, Ibaraki-ken 305–0901, Japan.
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- Takashi Awata
- Department of Animal Breeding and Genetics, National Institute of Animal Industry, Tsukuba Norin Danchi, Ibaraki-ken 305–0901, Japan.
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- Hirohumi Hanada
- Department of Animal Breeding and Genetics, National Institute of Animal Industry, Tsukuba Norin Danchi, Ibaraki-ken 305–0901, Japan.
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- Anthony C. F. Perry
- Laboratory of Developmental Biology, The Rockefeller University, New York, NY 10021, USA.
Description
<jats:p> Pig cloning will have a marked impact on the optimization of meat production and xenotransplantation. To clone pigs from differentiated cells, we microinjected the nuclei of porcine ( <jats:italic>Sus scrofa</jats:italic> ) fetal fibroblasts into enucleated oocytes, and development was induced by electroactivation. The transfer of 110 cloned embryos to four surrogate mothers produced an apparently normal female piglet. The clonal provenance of the piglet was indicated by her coat color and confirmed by DNA microsatellite analysis. </jats:p>
Journal
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- Science
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Science 289 (5482), 1188-1190, 2000-08-18
American Association for the Advancement of Science (AAAS)