PCR-Based Assay To Quantify Human Immunodeficiency Virus Type 1 DNA in Peripheral Blood Mononuclear Cells
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- Cindy Christopherson
- <!--label omitted: 1-->Roche Molecular Systems, Inc., Alameda, California 945011;
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- Yorda Kidane
- <!--label omitted: 1-->Roche Molecular Systems, Inc., Alameda, California 945011;
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- Brian Conway
- <!--label omitted: 2-->University of British Columbia2 and
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- John Krowka
- <!--label omitted: 4-->Viral and Rickettsial Disease Laboratory, Division of Communicable Disease Control, California Department of Health Services, Berkeley, California 947044
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- Haynes Sheppard
- <!--label omitted: 4-->Viral and Rickettsial Disease Laboratory, Division of Communicable Disease Control, California Department of Health Services, Berkeley, California 947044
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- Shirley Kwok
- <!--label omitted: 1-->Roche Molecular Systems, Inc., Alameda, California 945011;
説明
<jats:title>ABSTRACT</jats:title> <jats:p> An assay that quantifies the amount of human immunodeficiency virus type 1 (HIV-1) DNA in peripheral blood mononuclear cells has been developed. PCR amplification of the HIV-1 DNA is performed in the presence of an internal quantitation standard, and colorimetric detection of the amplified product is performed with microwell plates. The copies of HIV-1 DNA are normalized to total genomic DNA input. The assay has an analytical sensitivity of 10 input copies per amplification reaction and a three-log detection range. In an analysis of sequential samples from patients on combination therapy, HIV-1 DNA was quantifiable for all individuals tested, including those with undetectable plasma HIV-1 RNA. In a separate study, a comparison of HIV-1 DNA levels was made with a group of long-term survivors and progressors. The mean HIV-1 DNA levels were lower in the long-term survivors than in the progressors ( <jats:italic>P</jats:italic> , 0.04). The mean HIV-1 RNA levels were also lower, but the difference was not statistically significant ( <jats:italic>P</jats:italic> , 0.164). A quantitative DNA assay will provide an additional tool to gain insight into the natural history of infection and the continued efficacy of potent antiretroviral therapies. </jats:p>
収録刊行物
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- Journal of Clinical Microbiology
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Journal of Clinical Microbiology 38 (2), 630-634, 2000-02
American Society for Microbiology