Comparison of HgO and CuS04 as Digestion Catalysts in Manual Kjeldahl Determination of Crude Protein in Animal Feeds: Collaborative Study

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<jats:title>Abstract</jats:title> <jats:p>The official AOAC manual Kjeldahl method for determining crude protein in animal feeds, 7.015, uses HgO as a catalyst in the digestion step. Because of environmental considerations, there is considerable interest in alternative catalysts. A collaborative study compares the official HgO-catalyzed method and an alternative using CuS04. Fifty-four samples consisting of blind duplicates of closely matched pairs, representing a range of animal feed materials and 2 standard materials, were analyzed once by each method. Results were returned by 22 laboratories. Means and standard deviations between methods were comparable. The CuS04-catalyzed method has been adopted official first action.</jats:p>

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