Candidate reference methods for hemoglobin A1c based on peptide mapping

DOI PDF 被引用文献3件
  • Uwe Kobold
    Boehringer Mannheim GmbH Lab Diagnostics, Research Center Tutzing, Bahnhofstr. 9-15, D-82327 Tutzing, Germany
  • Jan-Olof Jeppsson
    Department of Clinical Chemistry, University of Lund, Malmö University Hospital, S-20502 Malmö, Sweden
  • Thomas Dülffer
    Boehringer Mannheim GmbH Lab Diagnostics, Research Center Tutzing, Bahnhofstr. 9-15, D-82327 Tutzing, Germany
  • Andreas Finke
    Boehringer Mannheim GmbH Lab Diagnostics, Research Center Tutzing, Bahnhofstr. 9-15, D-82327 Tutzing, Germany
  • Wieland Hoelzel
    Boehringer Mannheim GmbH Lab Diagnostics, Research Center Tutzing, Bahnhofstr. 9-15, D-82327 Tutzing, Germany
  • Kor Miedema
    De Weezenlanden Ziekenhuis, Groot Wezenland 20, NL-8000 GM Zwolle, The Netherlands

書誌事項

公開日
1997-10-01
権利情報
  • https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model
DOI
  • 10.1093/clinchem/43.10.1944
公開者
Oxford University Press (OUP)

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説明

<jats:title>Abstract</jats:title><jats:p>A reference method that specifically measures hemoglobin (Hb) A1c is an essential part of the reference system for the international standardization of Hb A1c/glycohemoglobin. We have developed a new method for quantification, based on the specific N-terminal residue of the hemoglobin β-chains. Enzymatic cleavage of the intact hemoglobin molecule with endoproteinase Glu-C has been optimized to obtain the β-N-terminal hexapeptides of Hb A1c and Hb A0. These peptides have been separated by reversed-phase HPLC and quantitated by electrospray ionization-mass spectrometry (method A) or by capillary electrophoresis (method B). With these peptides and hyphenated separation techniques, it has been possible to overcome the insufficient resolution of currently used protein separation systems for Hb A1c.</jats:p>

収録刊行物

  • Clinical Chemistry

    Clinical Chemistry 43 (10), 1944-1951, 1997-10-01

    Oxford University Press (OUP)

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