Heat Shock Cognate Protein 71‐Associated Peptides Function as an Epitope for <i>Toxoplasma gondii‐</i>Specific CD4<sup>+</sup> CTL

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  • Tian‐hui Yang
    Department of Parasitology Chiba University School of Medicine Chiba Chiba 260 Japan
  • Fumie Aosai
    Department of Parasitology Chiba University School of Medicine Chiba Chiba 260 Japan
  • Kazumi Norose
    Department of Parasitology Chiba University School of Medicine Chiba Chiba 260 Japan
  • Hye‐seong Mun
    Department of Parasitology Chiba University School of Medicine Chiba Chiba 260 Japan
  • Akihiko Yano
    Department of Parasitology Chiba University School of Medicine Chiba Chiba 260 Japan

書誌事項

公開日
1997-07
権利情報
  • http://onlinelibrary.wiley.com/termsAndConditions#vor
DOI
  • 10.1111/j.1348-0421.1997.tb01891.x
公開者
Wiley

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<jats:title>Abstract</jats:title><jats:p>HLA‐DR‐restricted CD4<jats:sup>+</jats:sup> cytotoxic T‐lymphocyte (CTL) lines specific for <jats:italic>Toxoplasma gondii</jats:italic> (<jats:italic>T. gondii</jats:italic>)‐infected melanoma cells have been established from peripheral blood lymphocytes (PBLs) of a patient with chronic toxoplasmosis. The role of heat shock cognate protein (HSC) 71 in antigen (Ag) processing and presentation of <jats:italic>T. gondii</jats:italic>‐infected melanoma cells to these CD4<jats:sup>+</jats:sup> CTL lines was investigated. A human melanoma cell line (P36) pulsed with <jats:italic>T. gondii‐</jats:italic>infected P36 cell‐derived HSC71 was lysed by a <jats:italic>T. gondii‐</jats:italic>specific CD4<jats:sup>+</jats:sup> CTL line (Tx‐HSC‐1). The Tx‐HSC‐1 also killed <jats:italic>T. gondii</jats:italic>‐infected P36 cells. The lytic activity of Tx‐HSC‐1 against P36 cells pulsed with <jats:italic>T. gondii</jats:italic>‐infected P36 cell‐derived HSC71 was inhibited by monoclonal antibodies (mAbs) against HSC71. Anti‐human leukocyte antigen (HLA)‐DR mAb also partially blocked the lytic activity, whereas anti‐HLA‐A,B,C mAb did not block the lytic activity. In addition, a flow cytometric analysis with these specific mAbs against HSC71 showed HSC71 to be expressed on the cell surface of <jats:italic>T. gondii</jats:italic>‐infected P36 cells as well as uninfected P36 cells. These data indicate that HSC71 molecules are expressed on human melanoma cell line P36, and that HSC71 may play a potential role in Ag presentation and processing of <jats:italic>T. gondii</jats:italic>‐infected P36 cells to CD4<jats:sup>+</jats:sup> CTL.</jats:p>

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