Recipient T Cells Mediate Reperfusion Injury after Lung Transplantation in the Rat

  • Marc de Perrot
    Thoracic Surgery Research Laboratory, Pathobiology and Immunology, Toronto General Hospital Research Institute, University of Toronto , Toronto ,
  • Kevin Young
    Departments of Laboratory Medicine, Pathobiology and Immunology, Toronto General Hospital Research Institute, University of Toronto , Toronto ,
  • Yumiko Imai
    Thoracic Surgery Research Laboratory, Pathobiology and Immunology, Toronto General Hospital Research Institute, University of Toronto , Toronto ,
  • Mingyao Liu
    Thoracic Surgery Research Laboratory, Pathobiology and Immunology, Toronto General Hospital Research Institute, University of Toronto , Toronto ,
  • Thomas K Waddell
    Thoracic Surgery Research Laboratory, Pathobiology and Immunology, Toronto General Hospital Research Institute, University of Toronto , Toronto ,
  • Stefan Fischer
    Thoracic Surgery Research Laboratory, Pathobiology and Immunology, Toronto General Hospital Research Institute, University of Toronto , Toronto ,
  • Li Zhang
    Departments of Laboratory Medicine, Pathobiology and Immunology, Toronto General Hospital Research Institute, University of Toronto , Toronto ,
  • Shaf Keshavjee
    Thoracic Surgery Research Laboratory, Pathobiology and Immunology, Toronto General Hospital Research Institute, University of Toronto , Toronto ,

書誌事項

公開日
2003-11
権利情報
  • https://academic.oup.com/pages/standard-publication-reuse-rights
DOI
  • 10.4049/jimmunol.171.10.4995
公開者
Oxford University Press (OUP)

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説明

<jats:title>Abstract</jats:title> <jats:p>Leukocytes have been implicated in ischemia-reperfusion (IR) injury of the lung, but the individual role of T cells has not been explored. Recent evidence in mice suggests that T cells may play a role in IR injury. Using a syngeneic (Lewis to Lewis) rat lung transplant model, we observed that recipient CD4+ T cells infiltrated lung grafts within 1 h of reperfusion and up-regulated the expression of CD25 over the ensuing 12 h. Nude rats (rnu/rnu) and heterozygous rats (rnu/+) were used to determine the role of T cells in IR injury. No significant difference in lung function was observed between nude and heterozygous recipient rats after 2 h of reperfusion. However, after 12 h of reperfusion, recipient nude rats had significantly higher oxygenation and lower peak airway pressure than recipient heterozygous rats. This was associated with significantly lower levels of IFN-γ in transplanted lung tissue of recipient nude rats. Reconstitution of recipient nude rats with T cells from heterozygous rats restored IR injury after 12 h of reperfusion. The effect of T cells was independent of neutrophil recruitment and activation in the transplanted lung. These results demonstrate that recipient T cells are activated and mediate IR injury during lung transplantation in rats.</jats:p>

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