Two DM domain genes, DMY and DMRT1, involved in testicular differentiation and development in the medaka, Oryzias latipes

Tohru Kobayashi, Masaru Matsuda, Hiroko Kajiura‐Kobayashi, Aya Suzuki, Noriko Saito, Masatoshi Nakamoto, Naoki Shibata, Yoshitaka Nagahama

doi:10.1002/dvdy.20158

<jats:title>Abstract</jats:title><jats:p>The recent discovery of the <jats:italic>DMY</jats:italic> gene (DM domain gene on Y chromosome and one of the <jats:italic>DMRT1</jats:italic> family genes) as a key determinant of male development in the medaka (<jats:italic>Oryzias latipes</jats:italic>) has led to its designation as the prime candidate gene for sex‐determination in this species. This study focused on the sites and pattern of expression of <jats:italic>DMY</jats:italic> and <jats:italic>DMRT1</jats:italic> genes during gonadal differentiation of medaka to further determine their roles in testis development. <jats:italic>DMY</jats:italic> mRNA and protein are expressed specifically in the somatic cells surrounding primordial germ cells (PGCs) in the early gonadal primordium, before morphological sex differences are seen. However, somatic cells surrounding PGCs never express <jats:italic>DMY</jats:italic> during the early migratory period. Expression of <jats:italic>DMY</jats:italic> persists in Sertoli cell lineage cells, from PGC‐supporting cells to Sertoli cells, indicating that only <jats:italic>DMY</jats:italic>‐positive cells enclose PGCs during mitotic arrest after hatching. <jats:italic>DMRT1</jats:italic> is expressed in spermatogonium‐supporting cells after testicular differentiation (20–30 days after hatching), and its expression is much higher than that of <jats:italic>DMY</jats:italic> in mature testes. In XX sex‐reversed testes, <jats:italic>DMRT1</jats:italic> is expressed in the Sertoli cell lineage, similar to the expression of <jats:italic>DMY</jats:italic> in XY testes. These results suggest strongly that <jats:italic>DMY</jats:italic> regulates PGC proliferation and differentiation sex‐specifically during early gonadal differentiation of XY individuals and that <jats:italic>DMRT1</jats:italic> regulates spermatogonial differentiation. Developmental Dynamics 231:518–526, 2004. © 2004 Wiley‐Liss, Inc.</jats:p>

Two DM domain genes, <i>DMY</i> and <i>DMRT1</i>, involved in testicular differentiation and development in the medaka, <i>Oryzias latipes</i>

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