Selective reduction of dormant maternal mRNAs in mouse oocytes by RNA interference

DOI PDF 被引用文献8件
  • Petr Svoboda
    University of Pennsylvania Department of Biology , , Philadelphia, PA 19104-6018, USA
  • Paula Stein
    University of Pennsylvania Department of Biology , , Philadelphia, PA 19104-6018, USA
  • Harutoshi Hayashi
    University of Pennsylvania Department of Biology , , Philadelphia, PA 19104-6018, USA
  • Richard M. Schultz
    University of Pennsylvania Department of Biology , , Philadelphia, PA 19104-6018, USA

説明

<jats:title>ABSTRACT</jats:title> <jats:p>Specific mRNA degradation mediated by double-stranded RNA (dsRNA), which is termed RNA interference (RNAi), is a useful tool with which to study gene function in several systems. We report here that in mouse oocytes, RNAi provides a suitable and robust approach to study the function of dormant maternal mRNAs. Mos (originally known as c-mos) and tissue plasminogen activator (tPA, Plat) mRNAs are dormant maternal mRNAs that are recruited during oocyte maturation; translation of Mos mRNA results in the activation of MAP kinase. dsRNA directed towards Mos or Plat mRNAs in mouse oocytes effectively results in the specific reduction of the targeted mRNA in both a time- and concentration-dependent manner. Moreover, dsRNA is more potent than either sense or antisense RNAs. Targeting the Mos mRNA results in inhibiting the appearance of MAP kinase activity and can result in parthenogenetic activation. Mos dsRNA, therefore, faithfully phenocopies the Mos null mutant. Targeting the Plat mRNA with Plat dsRNA results in inhibiting production of tPA activity. Finally, effective reduction of the Mos and Plat mRNA is observed with stoichiometric amounts of Mos and Plat dsRNA, respectively.</jats:p>

収録刊行物

  • Development

    Development 127 (19), 4147-4156, 2000-10-01

    The Company of Biologists

被引用文献 (8)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ