Human Osteoclast-Like Cells Are Formed from Peripheral Blood Mononuclear Cells in a Coculture with SaOS-2 Cells Transfected with the Parathyroid Hormone (PTH)/PTH-Related Protein Receptor Gene

DOI PDF PubMed 被引用文献2件 オープンアクセス
  • Kenichiro Matsuzaki
    Department of Biochemistry, School of Dentistry, Showa University (K.M., I.N., N.U., T.T., N.T., T.S.), 1–5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555
  • Kazuhiko Katayama
    Asahi Chemical Industry Company, Ltd., Laboratory for Bone Metabolism (K.K., Y.T., M.H.), 632–1 Mifuku, Ohito-cho, Tagata-gun, Shizuoka 410-2321
  • Yasuyuki Takahashi
    Asahi Chemical Industry Company, Ltd., Laboratory for Bone Metabolism (K.K., Y.T., M.H.), 632–1 Mifuku, Ohito-cho, Tagata-gun, Shizuoka 410-2321
  • Ichiro Nakamura
    Department of Biochemistry, School of Dentistry, Showa University (K.M., I.N., N.U., T.T., N.T., T.S.), 1–5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555
  • Nobuyuki Udagawa
    Department of Biochemistry, School of Dentistry, Showa University (K.M., I.N., N.U., T.T., N.T., T.S.), 1–5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555
  • Taro Tsurukai
    Department of Biochemistry, School of Dentistry, Showa University (K.M., I.N., N.U., T.T., N.T., T.S.), 1–5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555
  • Ryuichi Nishinakamura
    Institute of Medical Science University of Tokyo (R.N.), 4–6-1 Shiroganedai, Minato-ku, Tokyo 108-0071
  • Yoshiaki Toyama
    Department of Orthopaedic Surgery, School of Medicine, Keio University (K.M., Y.T., Y.Y.), 35 Shinanomachi, Shinjuku-ku, Tokyo 160-0016, Japan
  • Yutaka Yabe
    Department of Orthopaedic Surgery, School of Medicine, Keio University (K.M., Y.T., Y.Y.), 35 Shinanomachi, Shinjuku-ku, Tokyo 160-0016, Japan
  • Masayuki Hori
    Asahi Chemical Industry Company, Ltd., Laboratory for Bone Metabolism (K.K., Y.T., M.H.), 632–1 Mifuku, Ohito-cho, Tagata-gun, Shizuoka 410-2321
  • Naoyuki Takahashi
    Department of Biochemistry, School of Dentistry, Showa University (K.M., I.N., N.U., T.T., N.T., T.S.), 1–5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555
  • Tatsuo Suda
    Department of Biochemistry, School of Dentistry, Showa University (K.M., I.N., N.U., T.T., N.T., T.S.), 1–5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555

説明

Subclones of the human osteosarcoma cell line SaOS-2 were established by transfecting with an expression vector containing the human PTH/PTH-related protein (PTHrP) receptor, and their abilities to support osteoclast-like multinucleated cell (OCL) formation were examined in coculture with mouse or human hemopoietic cells. Of four subclones examined, SaOS-2/4 and SaOS-4/3 bound high levels of [125I]-PTH and produced a significant amount of cAMP in response to PTH. OCLs were formed in response to PTH in the cocultures of mouse bone marrow cells with either SaOS-2/4 cells or SaOS-4/3 cells. Human OCLs were also formed in response to PTH in the coculture of SaOS-4/3 cells and human peripheral blood mononuclear cells. Adding dexamethasone together with PTH greatly enhanced PTH-induced human OCL formation. Like mouse OCLs, human OCLs formed in response to PTH were tartrate-resistant acid phosphatase positive, expressed abundant calcitonin receptors and vitronectin receptors, and formed resorption pits on dentine slices. Other osteotropic factors such as 1alpha,25-dihydroxyvitamin D3, prostaglandin E2, and interleukin 6 plus soluble interleukin 6 receptors failed to induce mouse and human OCLs in cocultures with SaOS-4/3 cells. Both mouse and human OCL formation supported by SaOS-4/3 cells were inhibited by either adding an antibody against macrophage-colony stimulating factor or adding granulocyte/macrophage-colony stimulating factor. Thus, it is likely that human and mouse OCL formation supported by SaOS-4/3 cells are similarly regulated. These results indicate that the target cells of PTH for inducing osteoclast formation are osteoblast/stromal cells but not osteoclast progenitor cells in the coculture. This coculture model will be useful for investigating the abnormalities ofosteoclast differentiation and function in human metabolic bone diseases.

収録刊行物

  • Endocrinology

    Endocrinology 140 (2), 925-932, 1999-02-01

    The Endocrine Society

被引用文献 (2)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ