Application of D-Amino Acids as Biofilm Dispersing Agent in Dental Unit Waterlines

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  • Ruchanee Salingcarnboriboon Ampornaramveth
    Research Unit on Oral Microbiology and Immunology, Department of Microbiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand
  • Nilada Akeatichod
    Research Unit on Oral Microbiology and Immunology, Department of Microbiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand
  • Jesita Lertnukkhid
    Research Unit on Oral Microbiology and Immunology, Department of Microbiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand
  • Nichakorn Songsang
    Research Unit on Oral Microbiology and Immunology, Department of Microbiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand

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<jats:p><jats:italic>Aim and Purpose</jats:italic>. Biofilms in dental unit waterlines (DUWLs) are extremely difficult to eliminate. Aim of this study is to evaluate the efficacy of a mixture of four D-amino acids on biofilm dispersion in DUWLs.<jats:italic>Materials and Methods</jats:italic>. A mixture of four D-amino acids (D-methionine, D-tryptophan, D-leucine, and D-tyrosine, 10 mM each), distilled water (control), and 0.1 M hydrochloric acid (HCl) was used in the experiment. In laboratory, pieces of DUWLs covered with biofilms were submerged in different solutions for 5 days, flushed, and measured OD<jats:sup>600</jats:sup>of the dispersed biofilms. Remnants of biofilms on the DUWLs were evaluated by SEM. In clinic, fifteen DCUs were incubated with test and control solutions, flushed, and measured OD<jats:sup>600</jats:sup>of the dispersed biofilms. Microbial count of DUWL output water was enumerated twice a week for four weeks.<jats:italic>Results</jats:italic>. There was a slight, but not significant, increase in OD<jats:sup>600</jats:sup>of flushing water in D-amino acids group. D-amino acids effectively reduced bacterial plaque as demonstrated by SEM. Incubation with D-amino acids significantly reduced biofilms especially after the first day of flushing. Bacterial count in DUWL output water was significantly reduced after treatment with D-amino acids.<jats:italic>Conclusion</jats:italic>. D-amino acids are applicable as biofilm dispersing agents in DUWLs.</jats:p>

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