MHox: a mesodermally restricted homeodomain protein that binds an essential site in the muscle creatine kinase enhancer

  • Peter Cserjesi
    The University of Texas M. D. Anderson Cancer Center 1 Department of Biochemistry and Molecular Biology, Box 117 , , 1515 Holcombe Boulevard, Houston, TX 77030, USA
  • Brenda Lilly
    The University of Texas M. D. Anderson Cancer Center 1 Department of Biochemistry and Molecular Biology, Box 117 , , 1515 Holcombe Boulevard, Houston, TX 77030, USA
  • Laura Bryson
    The University of Texas M. D. Anderson Cancer Center 1 Department of Biochemistry and Molecular Biology, Box 117 , , 1515 Holcombe Boulevard, Houston, TX 77030, USA
  • Yaoqi Wang
    Boston University School of Medicine 2 Department of Biochemistry , , 80 East Concord Street, Boston, MA 02118, USA
  • David A. Sassoon
    Boston University School of Medicine 2 Department of Biochemistry , , 80 East Concord Street, Boston, MA 02118, USA
  • Eric N. Olson
    The University of Texas M. D. Anderson Cancer Center 1 Department of Biochemistry and Molecular Biology, Box 117 , , 1515 Holcombe Boulevard, Houston, TX 77030, USA

書誌事項

公開日
1992-08-01
権利情報
  • http://www.biologists.com/user-licence-1-1/
DOI
  • 10.1242/dev.115.4.1087
公開者
The Company of Biologists

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説明

<jats:title>ABSTRACT</jats:title> <jats:p>Myogenic helix-loop-helix (HLH) proteins, such as myo-genin and MyoD, can activate muscle-specific transcription when introduced into a variety of nonmuscle cell types. Whereas cells of mesodermal origin are especially permissive to the actions of these myogenic regulators, many other cell types are refractory to myogenic conversion by them. Here we describe a novel home-odomain protein, MHox, that binds an A+T-rich element in the muscle creatine kinase (MCK) enhancer that is essential for muscle-specific transcription and trans-activation by myogenic HLH proteins. MHox is completely restricted to mesodermally derived cell types during embryogenesis and to established cell lines of mesodermal origin. In contrast to most other homeobox genes, MHox expression is excluded from the nervous system, with the highest levels observed in limb bud and visceral arches. In adult mice, MHox is expressed at high levels in skeletal muscle, heart and uterus. The DNA-binding properties and pattern of MHox expression are unique among homeobox genes and suggest a role for MHox as a transcriptional regulator that participates in the establishment of diverse mesodermal cell types.</jats:p>

収録刊行物

  • Development

    Development 115 (4), 1087-1101, 1992-08-01

    The Company of Biologists

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