KaPPA-View. A Web-Based Analysis Tool for Integration of Transcript and Metabolite Data on Plant Metabolic Pathway Maps

  • Toshiaki Tokimatsu
    Kazusa DNA Research Institute, Kisarazu, Chiba 292–0818, Japan (T.T., N.S., H.S., K.S., D.S.); Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori, Yokohama 226–8501, Japan (H.O.); Graduate School of Life Sciences, Tohoku University, Aoba, Sendai 980–8578, Japan (K.N.); Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Aoba, Send
  • Nozomu Sakurai
    Kazusa DNA Research Institute, Kisarazu, Chiba 292–0818, Japan (T.T., N.S., H.S., K.S., D.S.); Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori, Yokohama 226–8501, Japan (H.O.); Graduate School of Life Sciences, Tohoku University, Aoba, Sendai 980–8578, Japan (K.N.); Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Aoba, Send
  • Hideyuki Suzuki
    Kazusa DNA Research Institute, Kisarazu, Chiba 292–0818, Japan (T.T., N.S., H.S., K.S., D.S.); Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori, Yokohama 226–8501, Japan (H.O.); Graduate School of Life Sciences, Tohoku University, Aoba, Sendai 980–8578, Japan (K.N.); Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Aoba, Send
  • Hiroyuki Ohta
    Kazusa DNA Research Institute, Kisarazu, Chiba 292–0818, Japan (T.T., N.S., H.S., K.S., D.S.); Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori, Yokohama 226–8501, Japan (H.O.); Graduate School of Life Sciences, Tohoku University, Aoba, Sendai 980–8578, Japan (K.N.); Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Aoba, Send
  • Kazuhiko Nishitani
    Kazusa DNA Research Institute, Kisarazu, Chiba 292–0818, Japan (T.T., N.S., H.S., K.S., D.S.); Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori, Yokohama 226–8501, Japan (H.O.); Graduate School of Life Sciences, Tohoku University, Aoba, Sendai 980–8578, Japan (K.N.); Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Aoba, Send
  • Tanetoshi Koyama
    Kazusa DNA Research Institute, Kisarazu, Chiba 292–0818, Japan (T.T., N.S., H.S., K.S., D.S.); Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori, Yokohama 226–8501, Japan (H.O.); Graduate School of Life Sciences, Tohoku University, Aoba, Sendai 980–8578, Japan (K.N.); Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Aoba, Send
  • Toshiaki Umezawa
    Kazusa DNA Research Institute, Kisarazu, Chiba 292–0818, Japan (T.T., N.S., H.S., K.S., D.S.); Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori, Yokohama 226–8501, Japan (H.O.); Graduate School of Life Sciences, Tohoku University, Aoba, Sendai 980–8578, Japan (K.N.); Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Aoba, Send
  • Norihiko Misawa
    Kazusa DNA Research Institute, Kisarazu, Chiba 292–0818, Japan (T.T., N.S., H.S., K.S., D.S.); Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori, Yokohama 226–8501, Japan (H.O.); Graduate School of Life Sciences, Tohoku University, Aoba, Sendai 980–8578, Japan (K.N.); Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Aoba, Send
  • Kazuki Saito
    Kazusa DNA Research Institute, Kisarazu, Chiba 292–0818, Japan (T.T., N.S., H.S., K.S., D.S.); Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori, Yokohama 226–8501, Japan (H.O.); Graduate School of Life Sciences, Tohoku University, Aoba, Sendai 980–8578, Japan (K.N.); Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Aoba, Send
  • Daisuke Shibata
    Kazusa DNA Research Institute, Kisarazu, Chiba 292–0818, Japan (T.T., N.S., H.S., K.S., D.S.); Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori, Yokohama 226–8501, Japan (H.O.); Graduate School of Life Sciences, Tohoku University, Aoba, Sendai 980–8578, Japan (K.N.); Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Aoba, Send

Search this article

Description

<jats:title>Abstract</jats:title> <jats:p>The application of DNA array technology and chromatographic separation techniques coupled with mass spectrometry to transcriptomic and metabolomic analyses in plants has resulted in the generation of considerable quantitative data related to transcription and metabolism. The integration of “omic” data is one of the major concerns associated with research into identifying gene function. Thus, we developed a Web-based tool, KaPPA-View, for representing quantitative data for individual transcripts and/or metabolites on plant metabolic pathway maps. We prepared a set of comprehensive metabolic pathway maps for Arabidopsis (Arabidopsis thaliana) and depicted these graphically in Scalable Vector Graphics format. Individual transcripts assigned to a reaction are represented symbolically together with the symbols of the reaction and metabolites on metabolic pathway maps. Using quantitative values for transcripts and/or metabolites submitted by the user as Comma Separated Value-formatted text through the Internet, the KaPPA-View server inserts colored symbols corresponding to a defined metabolic process at that site on the maps and returns them to the user's browser. The server also provides information on transcripts and metabolites in pop-up windows. To demonstrate the process, we describe the dataset obtained for transgenic plants that overexpress the PAP1 gene encoding a MYB transcription factor on metabolic pathway maps. The presentation of data in this manner is useful for viewing metabolic data in a way that facilitates the discussion of gene function.</jats:p>

Journal

  • Plant Physiology

    Plant Physiology 138 (3), 1289-1300, 2005-07-01

    Oxford University Press (OUP)

Citations (30)*help

See more

References(36)*help

See more

Related Data

See more

Related Projects

See more

Details 詳細情報について

Report a problem

Back to top