Sequences Necessary for Nitrate-Dependent Transcription of Arabidopsis Nitrate Reductase Genes

DOI PDF 被引用文献8件
  • C. F. Hwang
    Department of Biological Sciences, The University of Iowa, Iowa City, Iowa 52242
  • Y. Lin
    Department of Biological Sciences, The University of Iowa, Iowa City, Iowa 52242
  • T. D'Souza
    Department of Biological Sciences, The University of Iowa, Iowa City, Iowa 52242
  • C. L. Cheng
    Department of Biological Sciences, The University of Iowa, Iowa City, Iowa 52242

説明

<jats:title>Abstract</jats:title> <jats:p>Nitrate increases the transcription of the two Arabidopsis thaliana nitrate reductase genes. We demonstrated previously that 238 and 330 bp of the 5[prime] flanking regions, designated as NP1 and NP2, of the two nitrate reductase genes NR1 and NR2, respectively, are sufficient for nitrate-dependent transcription (Y. Lin, C.-F. Hwang, J.B. Brown, C.-L. Cheng [1994] Plant Physiol 106: 477–484). Here we identify the cis-acting elements of NP1 and NP2 that are necessary for nitrate-dependent transcription by linker-scanning (LS) analysis. In transgenic plants one LS mutant of NP1 and two LS mutants of NP2 exhibited significantly lower nitrate-induced reporter gene chloramphenicol acetyltransferase activity. To distinguish which of these three mutants lost nitrate inducibility, competitive reverse-transcriptase polymerase chain reaction was used to measure the chloramphenicol acetyltransferase mRNA levels before and after nitrate induction. The single LS mutant in NP1 lost its response to nitrate, whereas the two LS mutants in NP2 partially lost their response to nitrate. A 12-bp sequence is conserved between the NP1 site and the two NP2 sites. This sequence motif is also conserved in the 5[prime] flanking regions of other nitrate-inducible plant genes. Gel mobility shift experiments indicate that these three regions bind to similar proteins. The binding is constitutive with respect to nitrate treatment and was observed in both nonphotosynthetic suspension cells and green leaves.</jats:p>

収録刊行物

  • Plant Physiology

    Plant Physiology 113 (3), 853-862, 1997-03-01

    Oxford University Press (OUP)

被引用文献 (8)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ