Distinct Roles for Cyclin-Dependent Kinases in Cell Cycle Control

DOI Web Site 10 Citations
  • Sander van den Heuvel
    Massachusetts General Hospital Cancer Center, Charlestown, MA 02129.
  • Ed Harlow
    Massachusetts General Hospital Cancer Center, Charlestown, MA 02129.

Abstract

<jats:p> The key cell-cycle regulator Cdc2 belongs to a family of cyclin-dependent kinases in higher eukaryotes. Dominant-negative mutations were used to address the requirement for kinases of this family in progression through the human cell cycle. A dominant-negative Cdc2 mutant arrested cells at the G <jats:sub>2</jats:sub> to M phase transition, whereas mutants of the cyclin-dependent kinases Cdk2 and Cdk3 caused a G <jats:sub>1</jats:sub> block. The mutant phenotypes were specifically rescued by the corresponding wild-type kinases. These data reveal that Cdk3, in addition to Cdc2 and Cdk2, executes a distinct and essential function in the mammalian cell cycle. </jats:p>

Journal

  • Science

    Science 262 (5142), 2050-2054, 1993-12-24

    American Association for the Advancement of Science (AAAS)

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