Use of the Fluidigm C1 platform for RNA sequencing of single mouse pancreatic islet cells

DOI Web Site 5 Citations

Description

<jats:title>Significance</jats:title> <jats:p>Pancreatic islets are complex structures composed of four cell types whose primary function is to maintain glucose homeostasis. Owing to the scarcity and heterogeneity of the islet cell types, little is known about their individual gene expression profiles. Here we used the Fluidigm C1 platform to obtain high-quality gene expression profiles of each islet cell type from mice. We identified cell-type–specific transcription factors and pathways providing previously unrecognized insights into genes characterizing islet cells. Unexpectedly, our data uncover technical limitations with the C1 Fluidigm cell capture process, which should be considered when analyzing single-cell transcriptomics data.</jats:p>

Journal

Citations (5)*help

See more

Details 詳細情報について

Report a problem

Back to top