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The monoclonal antibody specific for the 4‐hydroxy‐2‐nonenal histidine adduct
Bibliographic Information
- Published
- 1995-02-13
- Rights Information
-
- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
-
- 10.1016/0014-5793(95)00033-6
- Publisher
- Wiley
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Description
<jats:p>Monoclonal antibodies directed against proteins modified with the major membrane lipid peroxidation product, 4‐hydroxy‐2‐nonenal, have been established and characterized. The monoclonal antibodies specific for HNE‐modified proteins were raised by immunizing mice with a HNE‐keyhole limpet hemocyanin conjugate. The resulting five monoclonal antibodies (mAbs HNEJ‐1–5) recognized HNE‐modified bovine serum albumin (BSA), but not native BSA in Western blot studies. Of the five mAbs, HNEJ‐2 exhibited the highest affinity for HNE‐modified proteins and a much higher affinity for the HNE‐histidine adduct than the HNE‐lysine or HNE‐cysteine adducts. mAb HNEJ‐2 did not cross‐react with proteins that had been treated with other aldehydes, such as 1‐hexenal, 2‐hexenal, 4‐hydroxy‐2‐hexenal, 2‐nonenal, formaldehyde, or glutaraldehyde. These results suggest that the major epitope recognized by mAb HNEJ‐2 is the Michael addition‐type HNE‐histidine adduct.</jats:p>
Journal
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- FEBS Letters
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FEBS Letters 359 (2-3), 189-191, 1995-02-13
Wiley
