Two-Step Regulation of Left–Right Asymmetric Expression of Pitx2
説明
Pitx2 is left--right (L--R) asymmetrically expressed initially in the lateral plate and later in primordial visceral organs. The transcriptional regulatory mechanisms that underlie L--R asymmetric expression of Pitx2 were investigated. Mouse Pitx2 has a left side-specific enhancer (ASE) that mediates both the initiation and maintenance of L--R asymmetric expression. This element contains three binding sites for the transcription factor FAST. The FAST binding sites function as Nodal-responsive elements and are sufficient for the initiation but not for the maintenance of asymmetric expression. The maintenance requires an Nkx2-5 binding site also present within the ASE. These results suggest that the left-sided expression of Pitx2 is directly initiated by Nodal signaling and is subsequently maintained by Nkx2. Such two-step control may represent a general mechanism for gene regulation during development.
収録刊行物
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- Molecular Cell
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Molecular Cell 7 (1), 137-149, 2001-01
Elsevier BV
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キーワード
- Nodal Protein
- Left-Right Determination Factors
- Xenopus
- Molecular Sequence Data
- Xenopus Proteins
- Mice
- Transforming Growth Factor beta
- Homeobox Protein PITX2
- Animals
- Paired Box Transcription Factors
- Transgenes
- Molecular Biology
- Body Patterning
- Homeodomain Proteins
- Binding Sites
- Base Sequence
- Gene Expression Regulation, Developmental
- Nuclear Proteins
- Forkhead Transcription Factors
- Cell Biology
- DNA-Binding Proteins
- Enhancer Elements, Genetic
- Lac Operon
- Homeobox Protein Nkx-2.5
- Signal Transduction
- Transcription Factors
詳細情報 詳細情報について
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- CRID
- 1363951795033869056
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- NII論文ID
- 30010673881
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- ISSN
- 10972765
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- PubMed
- 11172719
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- データソース種別
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- Crossref
- CiNii Articles
- OpenAIRE