Mobilization of Endothelial Progenitor Cells in Patients With Acute Myocardial Infarction

DOI Web Site 被引用文献38件
  • Satoshi Shintani
    From the Cardiovascular Research Institute and Department of Internal Medicine III, Kurume University, and the Institute of Molecular Embryology and Genetics, Kumamoto University (Y.O.), Japan.
  • Toyoaki Murohara
    From the Cardiovascular Research Institute and Department of Internal Medicine III, Kurume University, and the Institute of Molecular Embryology and Genetics, Kumamoto University (Y.O.), Japan.
  • Hisao Ikeda
    From the Cardiovascular Research Institute and Department of Internal Medicine III, Kurume University, and the Institute of Molecular Embryology and Genetics, Kumamoto University (Y.O.), Japan.
  • Takafumi Ueno
    From the Cardiovascular Research Institute and Department of Internal Medicine III, Kurume University, and the Institute of Molecular Embryology and Genetics, Kumamoto University (Y.O.), Japan.
  • Tomoki Honma
    From the Cardiovascular Research Institute and Department of Internal Medicine III, Kurume University, and the Institute of Molecular Embryology and Genetics, Kumamoto University (Y.O.), Japan.
  • Atsushi Katoh
    From the Cardiovascular Research Institute and Department of Internal Medicine III, Kurume University, and the Institute of Molecular Embryology and Genetics, Kumamoto University (Y.O.), Japan.
  • Ken-ichiro Sasaki
    From the Cardiovascular Research Institute and Department of Internal Medicine III, Kurume University, and the Institute of Molecular Embryology and Genetics, Kumamoto University (Y.O.), Japan.
  • Toshifumi Shimada
    From the Cardiovascular Research Institute and Department of Internal Medicine III, Kurume University, and the Institute of Molecular Embryology and Genetics, Kumamoto University (Y.O.), Japan.
  • Yuichi Oike
    From the Cardiovascular Research Institute and Department of Internal Medicine III, Kurume University, and the Institute of Molecular Embryology and Genetics, Kumamoto University (Y.O.), Japan.
  • Tsutomu Imaizumi
    From the Cardiovascular Research Institute and Department of Internal Medicine III, Kurume University, and the Institute of Molecular Embryology and Genetics, Kumamoto University (Y.O.), Japan.

抄録

<jats:p> <jats:italic>Background</jats:italic> —Endothelial progenitor cells (EPCs) circulate in adult peripheral blood (PB) and contribute to neovascularization. However, little is known regarding whether EPCs and their putative precursor, CD34-positive mononuclear cells (MNC <jats:sup>CD34+</jats:sup> ), are mobilized into PB in acute ischemic events in humans. </jats:p> <jats:p> <jats:italic>Methods and Results</jats:italic> —Flow cytometry revealed that circulating MNC <jats:sup>CD34+</jats:sup> counts significantly increased in patients with acute myocardial infarction (n=16), peaking on day 7 after onset, whereas they were unchanged in control subjects (n=8) who had no evidence of cardiac ischemia. During culture, PB-MNCs formed multiple cell clusters, and EPC-like attaching cells with endothelial cell lineage markers (CD31, vascular endothelial cadherin, and kinase insert domain receptor) sprouted from clusters. In patients with acute myocardial infarction, more cell clusters and EPCs developed from cultured PB-MNCs obtained on day 7 than those on day 1. Plasma levels of vascular endothelial growth factor significantly increased, peaking on day 7, and they positively correlated with circulating MNC <jats:sup>CD34+</jats:sup> counts ( <jats:italic>r</jats:italic> =0.35, <jats:italic>P</jats:italic> =0.01). </jats:p> <jats:p> <jats:italic>Conclusions</jats:italic> —This is the first clinical demonstration showing that lineage-committed EPCs and MNC <jats:sup>CD34+</jats:sup> , their putative precursors, are mobilized during an acute ischemic event in humans. </jats:p>

収録刊行物

  • Circulation

    Circulation 103 (23), 2776-2779, 2001-06-12

    Ovid Technologies (Wolters Kluwer Health)

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