Synthetic evolutionary origin of a proofreading reverse transcriptase

Jared W. Ellefson, Jimmy Gollihar, Raghav Shroff, Haridha Shivram, Vishwanath R. Iyer, Andrew D. Ellington

doi:10.1126/science.aaf5409

Synthetic evolutionary origin of a proofreading reverse transcriptase

DOI Web Site 被引用文献6件

Jared W. Ellefson

Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, Department of Molecular Biosciences, University of Texas, 2500 Speedway, Austin, TX 78712, USA.
Jimmy Gollihar

Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, Department of Molecular Biosciences, University of Texas, 2500 Speedway, Austin, TX 78712, USA.
Raghav Shroff

Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, Department of Molecular Biosciences, University of Texas, 2500 Speedway, Austin, TX 78712, USA.
Haridha Shivram

Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, Department of Molecular Biosciences, University of Texas, 2500 Speedway, Austin, TX 78712, USA.
Vishwanath R. Iyer

Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, Department of Molecular Biosciences, University of Texas, 2500 Speedway, Austin, TX 78712, USA.
Andrew D. Ellington

Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, Department of Molecular Biosciences, University of Texas, 2500 Speedway, Austin, TX 78712, USA.

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<jats:title>Making error-free DNA from RNA</jats:title> <jats:p> DNA polymerase enzymes copy DNA into new strands of identical DNA. Reverse transcriptase (RT) enzymes copy RNA into DNA. Unlike many DNA polymerases, RT enzymes do not have a proofreading function that checks for errors in the newly synthesized DNA. Ellefson <jats:italic>et al.</jats:italic> use in vitro directed evolution and protein engineering to build an error-correcting RT from a prokaryotic DNA polymerase. The RT “xenopolymerase” shows increased fidelity as compared to natural RTs and should streamline and increase the precision of transcriptomics methods. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6293" page="1590" related-article-type="in-this-issue" vol="352" xlink:href="10.1126/science.aaf5409">1590</jats:related-article> </jats:p>