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G Protein Signaling from Activated Rat Frizzled-1 to the β-Catenin-Lef-Tcf Pathway
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- Tong Liu
- Department of Molecular Pharmacology and
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- Anthony J. DeCostanzo
- Department of Molecular Pharmacology and
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- Xunxian Liu
- Department of Molecular Pharmacology and
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- Hsien-yu Wang
- Department of Physiology, Diabetes and Metabolic Diseases Research Center, University Medical Center, State University of New York at Stony Brook, Stony Brook, NY 11794–8651, USA.
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- Sarah Hallagan
- Howard Hughes Medical Institute, Department of Pharmacology and Center for Developmental Biology, University of Washington School of Medicine, Seattle, WA 98195, USA.
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- Randall T. Moon
- Howard Hughes Medical Institute, Department of Pharmacology and Center for Developmental Biology, University of Washington School of Medicine, Seattle, WA 98195, USA.
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- Craig C. Malbon
- Department of Molecular Pharmacology and
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Description
<jats:p> The <jats:italic>frizzled</jats:italic> receptors, which mediate development and display seven hydrophobic, membrane-spanning segments, are cell membrane–localized. We constructed a chimeric receptor with the ligand-binding and transmembrane segments from the β <jats:sub>2</jats:sub> -adrenergic receptor (β <jats:sub>2</jats:sub> AR) and the cytoplasmic domains from rat Frizzled-1 (Rfz1). Stimulation of mouse F9 clones expressing the chimera (β <jats:sub>2</jats:sub> AR-Rfz1) with the β-adrenergic agonist isoproterenol stimulated stabilization of β-catenin, activation of a β-catenin–sensitive promoter, and formation of primitive endoderm. The response was blocked by inactivation of pertussis toxin–sensitive, heterotrimeric guanine nucleotide–binding proteins (G proteins) and by depletion of Gαq and Gαo. Thus, G proteins are elements of Wnt/Frizzled-1 signaling to the β-catenin–lymphoid-enhancer factor (LEF)-T cell factor (Tcf) pathway. </jats:p>
Journal
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- Science
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Science 292 (5522), 1718-1722, 2001-06
American Association for the Advancement of Science (AAAS)
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Details 詳細情報について
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- CRID
- 1364233268717492608
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- NII Article ID
- 30020378147
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- ISSN
- 10959203
- 00368075
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- Data Source
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- Crossref
- CiNii Articles