Towards clinical proteomics analysis

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<jats:title>Abstract</jats:title><jats:p>To understand accurate protein dynamics, a highly reproducible proteomics analytical method is required. The acquired thus knowledge will lead to the diagnosis, treatment and protection against diseases. This review deals with proteomics analysis from a view of sample pre‐treatment, sensitivity and reproducibility of the methods, including two‐dimensional polyacrylamide gel electrophoresis (2D‐PAGE), liquid chromatography–tandem mass spectrometry (LC‐MS/MS). and fluorogenic derivatization (FD)‐LC‐MS/MS. Copyright © 2010 John Wiley & Sons, Ltd.</jats:p>

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