Complete Structure of the Enterococcal Polysaccharide Antigen (EPA) of Vancomycin-Resistant Enterococcus faecalis V583 Reveals that EPA Decorations Are Teichoic Acids Covalently Linked to a Rhamnopolysaccharide Backbone
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- Yann Guerardel
- Univ. Lille, CNRS, UMR 8576—UGSF—Unité de Glycobiologie Structurale et Fonctionnelle, Lille, France
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- Irina Sadovskaya
- Univ. Littoral Côte d’Opale, UMR 1158 BioEcoAgro, TERRA Viollette, USC Anses, INRAe, Univ. Lille, Univ. Artois, Univ. Picardie Jules Verne, Univ. Liège, Yncréa, Boulogne-sur-Mer, France
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- Emmanuel Maes
- Univ. Lille, CNRS, UMR 8576—UGSF—Unité de Glycobiologie Structurale et Fonctionnelle, Lille, France
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- Sylviane Furlan
- Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France
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- Marie-Pierre Chapot-Chartier
- Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France
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- Stéphane Mesnage
- University of Sheffield, Department of Molecular Biology and Biotechnology, Sheffield, United Kingdom
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- Lionel Rigottier-Gois
- Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France
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- Pascale Serror
- Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France
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- Gary M. Dunny
- editor
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説明
<jats:p> Enterococci are opportunistic pathogens responsible for hospital- and community-acquired infections. All enterococci produce a surface polysaccharide called EPA ( <jats:italic>e</jats:italic> nterococcal <jats:italic>p</jats:italic> olysaccharide <jats:italic>a</jats:italic> ntigen) required for biofilm formation, antibiotic resistance, and pathogenesis. Despite the critical role of EPA in cell growth and division and as a major virulence factor, no information is available on its structure. Here, we report the complete structure of the EPA polymer produced by the model strain <jats:named-content content-type="genus-species">E. faecalis</jats:named-content> V583. We describe the structure of the EPA backbone, made of a rhamnan hexasaccharide substituted by Glc and GlcNAc residues, and show that teichoic acids are covalently bound to this rhamnan chain, forming the so-called “EPA decorations” essential for host colonization and pathogenesis. This report represents a key step in efforts to identify the structural properties of EPA that are essential for its biological activity and to identify novel targets to develop preventive and therapeutic approaches against enterococci. </jats:p>
収録刊行物
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- mBio
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mBio 11 (2), e00277-20-, 2020-04-28
American Society for Microbiology
